PMID- 34211000
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210930
LR  - 20240402
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jul 1
TI  - A sample cell for the study of enzyme-induced carbonate precipitation at the 
      grain-scale and its implications for biocementation.
PG  - 13675
LID - 10.1038/s41598-021-92235-7 [doi]
LID - 13675
AB  - Biocementation is commonly based on microbial-induced carbonate precipitation 
      (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization 
      of [Formula: see text] in a granular medium is used to produce a sustainable, 
      consolidated porous material. The successful implementation of biocementation in 
      large-scale applications requires detailed knowledge about the micro-scale 
      processes of [Formula: see text] precipitation and grain consolidation. For this 
      purpose, we present a microscopy sample cell that enables real time and in situ 
      observations of the precipitation of [Formula: see text] in the presence of sand 
      grains and calcite seeds. In this study, the sample cell is used in combination 
      with confocal laser scanning microscopy (CLSM) which allows the monitoring in 
      situ of local pH during the reaction. The sample cell can be disassembled at the 
      end of the experiment, so that the precipitated crystals can be characterized 
      with Raman microspectroscopy and scanning electron microscopy (SEM) without 
      disturbing the sample. The combination of the real time and in situ monitoring of 
      the precipitation process with the possibility to characterize the precipitated 
      crystals without further sample processing, offers a powerful tool for 
      knowledge-based improvements of biocementation.
FAU - Zehner, Jennifer
AU  - Zehner J
AD  - Department of Physics, Norwegian University of Science and Technology, Trondheim, 
      Norway. jennifer.s.zehner@ntnu.no.
FAU - Royne, Anja
AU  - Royne A
AD  - The Njord Centre, Department of Physics, University of Oslo, Oslo, Norway.
FAU - Sikorski, Pawel
AU  - Sikorski P
AD  - Department of Physics, Norwegian University of Science and Technology, Trondheim, 
      Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210701
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
PMC - PMC8249643
COIS- The authors declare no competing interests.
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
PMCR- 2021/07/01
CRDT- 2021/07/02 06:05
PHST- 2020/11/01 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/07/02 06:05 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
PHST- 2021/07/01 00:00 [pmc-release]
AID - 10.1038/s41598-021-92235-7 [pii]
AID - 92235 [pii]
AID - 10.1038/s41598-021-92235-7 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jul 1;11(1):13675. doi: 10.1038/s41598-021-92235-7.