PMID- 34212548
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210707
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 13
IP  - 4
DP  - 2021 Jul
TI  - Allergenicity and Stability of 6 New Korean Bony Fish Extracts.
PG  - 623-637
LID - 10.4168/aair.2021.13.4.623 [doi]
AB  - PURPOSE: Diagnostic tests for allergen sensitization should reflect real 
      exposure. We made 6 new bony fish extracts, which are consumed popularly in 
      Korea, and evaluated their allergenicity and stability. METHODS: We manufactured 
      fish extracts from codfish, mackerel, common eel, flounder, cutlass, and catfish. 
      Protein and parvalbumin (PV) were evaluated by Bradford assay, 2-site 
      enzyme-linked immunosorbent assay, sodium dodecyl sulphate-polyacrylamide gel 
      electrophoresis (SDS-PAGE), and anti-PV immunoblotting. The immunoglobulin E 
      (IgE) reactivities of the extracts were evaluated with ImmunoCAP and IgE 
      immunoblotting using sera from 24 Korean fish allergy patients, 5 asymptomatic 
      sensitizers, and 11 non-atopic subjects. Stability of the extracts stored in 4 
      different buffers were evaluated for up to a year. RESULTS: The protein 
      concentrations of commercial SPT fish extracts varied with up to a 7.5-fold 
      difference. SDS-PAGE showed marked differences in the PV concentrations of 
      commercial SPT reagents. Specific IgE measurements for the following 
      investigatory fish extracts-iCodfish, iMackerel, and iEel-were concordant with 
      that of their corresponding Phadia ImmunoCAP measurements. ImmunoCAP results 
      showed marked IgE cross-reactivity among the fish species, and the overall 
      sensitivity of ImmunoCAP with the investigatory fish extracts for identification 
      of culprit fish species was 85.7%. The protein and PV concentrations in the 
      investigatory extracts were highly stable in saline with 0.3% phenol-50% glycerol 
      at 4 degrees C for up to a year. CONCLUSIONS: The commercial SPT fish extracts exhibited 
      considerable variation in terms of allergenicity, which may impact on diagnostic 
      accuracy. Our new fish extracts have sufficient allergenicity and stability and 
      may be adequate to various clinical applications.
CI  - Copyright (c) 2021 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Yuk, Ji Eun
AU  - Yuk JE
AUID- ORCID: 0000-0003-2517-2653
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Jongsun
AU  - Lee J
AUID- ORCID: 0000-0003-1526-2241
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AUID- ORCID: 0000-0001-9887-1426
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Park, Kyung Hee
AU  - Park KH
AUID- ORCID: 0000-0003-3605-5364
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Kim, Jung Dong
AU  - Kim JD
AUID- ORCID: 0000-0002-6348-9267
AD  - Prolagen, Seoul, Korea.
FAU - Kim, Ji Tae
AU  - Kim JT
AUID- ORCID: 0000-0002-8690-358X
AD  - Prolagen, Seoul, Korea.
FAU - Lee, Jae Hyun
AU  - Lee JH
AUID- ORCID: 0000-0002-0760-0071
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
FAU - Park, Jung Won
AU  - Park JW
AUID- ORCID: 0000-0003-0249-8749
AD  - Division of Allergy and Immunology, Department of Internal Medicine, Institute of 
      Allergy, Yonsei University College of Medicine, Seoul, Korea.
AD  - Prolagen, Seoul, Korea. parkjw@yuhs.ac.
LA  - eng
GR  - 20006057/MOTIE/Ministry of Trade, Industry and Energy/Korea
GR  - HI14C1324/Korea Health Industry Development Institute/Republic of Korea
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC8255345
OTO - NOTNLM
OT  - Allergenicity
OT  - bony fish
OT  - extracts
OT  - fish allergy
OT  - fish parvalbumin
OT  - food allergy
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
PMCR- 2021/07/01
CRDT- 2021/07/02 07:08
PHST- 2020/07/28 00:00 [received]
PHST- 2020/11/04 00:00 [revised]
PHST- 2020/12/14 00:00 [accepted]
PHST- 2021/07/02 07:08 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
PHST- 2021/07/01 00:00 [pmc-release]
AID - 13.623 [pii]
AID - 10.4168/aair.2021.13.4.623 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2021 Jul;13(4):623-637. doi: 
      10.4168/aair.2021.13.4.623.