PMID- 34213105
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210702
LR  - 20210702
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 11
DP  - 2020 Nov 8
TI  - [Simultaneous and rapid determination of malachite green and leucomalachite green 
      by a label-free colorimetric aptasensor].
PG  - 1332-1339
LID - 10.3724/SP.J.1123.2020.04010 [doi]
AB  - A label-free colorimetric aptasensor, using a bispecific aptamer (A3) as a 
      sensing probe, gold nanoparticles (AuNPs) as an indicator, and NaCl solution as 
      an aggregation inducer, was successfully developed for the simultaneous, rapid 
      and visual detection of malachite green (MG) and leucomalachite green (LMG) in 
      aquatic products. This method is based on the aptamer A3 having bispecific 
      binding ability with MG and LMG, making it an ideal recognition receptor for MG 
      and LMG. It can adsorb on the AuNPs and protect AuNPs against salt-induced 
      aggregation, maintaining the red color of the solution. When MG or LMG was added 
      to a solution, aggregation of AuNPs was specifically induced by desorption of 
      aptamer from the AuNPs surface upon formation of the aptamer-target complex. 
      Therefore, the salt could trigger aggregation of AuNPs and the solution color was 
      changed from red to blue. This color change allowed the qualitative determination 
      of MG and LMG visually, and quantitative determination by measuring the ratio of 
      the absorbances at 520 nm and 650 nm. In this study, 50 muL of the nucleic acid 
      aptamer A3 (final concentrations 150 nmol/L) and 150 muL of AuNPs (final 
      concentrations 1.25 nmol/L) were incubated at room temperature (RT) for 6 min, 
      then 50 muL of the sample was added and incubated at RT for 30 min, and finally 50 
      muL NaCl solution (final concentrations 150 mmol/L) was added. After 4 min, the 
      solution color change was observed, and the absorbances at 520 nm and 650 nm were 
      measured. Under the optimal conditions, MG and LMG could be detected specifically 
      without any cross-reactivity with sulfadiazine (SDZ) and nitrofurantoin (NFT). 
      The absorbance were related to the concentrations of MG and LMG, and a good 
      linear relationship was obtained in the range of 0-17.5 mumol/L. The correlation 
      coefficients (R(2)) were 0.9938 and 0.9715, respectively. The limits of detection 
      of MG and LMG were 6.93 nmol/L and 6.38 nmol/L, respectively. The spiked 
      recoveries of MG and LMG ranged from 88.60% to 93.30% and 101.80% to 107.00%, 
      respectively. The relative standard deviations (RSDs) of MG and LMG ranged from 
      2.27% to 3.55% and 2.62% to 3.75%, respectively. This colorimetric method is 
      simple, rapid, sensitive, and allows visual, and it can provide a new method for 
      the simultaneous and rapid determination of the MG and LMG in aquatic products.
FAU - Wu, Wenwei
AU  - Wu W
AD  - Agricultural College of Yanbian University, Yanji 133002, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - College of Science of Beihua University, Jilin 132001, China.
FAU - Liu, Kexin
AU  - Liu K
AD  - Agricultural College of Yanbian University, Yanji 133002, China.
FAU - Li, Tiansong
AU  - Li T
AD  - College of Science of Beihua University, Jilin 132001, China.
FAU - Yang, Yongjie
AU  - Yang Y
AD  - Agricultural College of Yanbian University, Yanji 133002, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
SB  - IM
OTO - NOTNLM
OT  - bispecific aptamer
OT  - gold nanoparticles (AuNPs) colorimetry
OT  - leucomalachite Green (LMG)
OT  - malachite Green (MG)
OT  - simultaneous and rapid detection
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
CRDT- 2021/07/02 09:12
PHST- 2021/07/02 09:12 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
AID - 10.3724/SP.J.1123.2020.04010 [doi]
PST - ppublish
SO  - Se Pu. 2020 Nov 8;38(11):1332-1339. doi: 10.3724/SP.J.1123.2020.04010.