PMID- 34213136
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210702
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 12
DP  - 2019 Dec 8
TI  - [Determination of indoles in plasma of polycystic ovarian syndrome patients by 
      high performance liquid chromatography-fluorescence detection].
PG  - 1337-1342
LID - 10.3724/SP.J.1123.2019.05039 [doi]
AB  - A new method was established for the determination of indoles (indole (IND), 
      3-methylindole (3-MI), indolyl-3-acetic acid (IAA) and indolyl-3-propionic acid 
      (IPA)) in plasma by high performance liquid chromatography-fluorescence detection 
      (HPLC-FLD). The analytes were separated simultaneously on a Shim-pack VP-ODS 
      column (150 mmx4.6 mm, 4.6 mum) using 15 mmol/L sodium dihydrogen phosphate 
      solution and methanol (48:52, v/v) as the mobile phases. The column temperature 
      was 30 ℃, and the flow rate was 0.8 mL/min. The calibration curves of IND, 3-MI, 
      IPA and IAA showed good linear relationships. The intra-day and inter-day 
      relative standard deviations (RSDs) for the analytes were both less than 6.31%. 
      The average recoveries of the analytes in plasma were 97.5%-107.0%. The method 
      was successfully applied to the analysis of indoles in the plasma of healthy 
      women of reproductive age (n=25) as controls and patients with polycystic ovarian 
      syndrome (n=61). The results showed that the concentrations of indoles in the 
      plasma were significantly different between the two groups, and IND was found to 
      be a risk factor and a potential diagnostic biomarker for polycystic ovarian 
      syndrome (PCOS). The method is simple, sensitive and suitable for use in clinical 
      testing and laboratory research.
FAU - Han, Runchuan
AU  - Han R
AD  - Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), 
      School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, 
      China.
FAU - Ban, Yanna
AU  - Ban Y
AD  - Reproductive Health and Infertility Department the First Affiliated Hospital of 
      Chongqing Medical University, Chongqing 400016, China.
FAU - Zhen, Qianna
AU  - Zhen Q
AD  - Endocrinology Department, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, China.
FAU - Zhou, Ying
AU  - Zhou Y
AD  - Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), 
      School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, 
      China.
FAU - Zhang, Xiaoqing
AU  - Zhang X
AD  - Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), 
      School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, 
      China.
FAU - Ding, Min
AU  - Ding M
AD  - Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), 
      School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
SB  - IM
OTO - NOTNLM
OT  - high performance liquid chromatography-fluorescence detection (HPLC-FLD)
OT  - indoles
OT  - plasma
OT  - polycystic ovarian syndrome (PCOS)
EDAT- 2019/12/08 00:00
MHDA- 2019/12/08 00:01
CRDT- 2021/07/02 09:12
PHST- 2021/07/02 09:12 [entrez]
PHST- 2019/12/08 00:00 [pubmed]
PHST- 2019/12/08 00:01 [medline]
AID - 10.3724/SP.J.1123.2019.05039 [doi]
PST - ppublish
SO  - Se Pu. 2019 Dec 8;37(12):1337-1342. doi: 10.3724/SP.J.1123.2019.05039.