PMID- 34213170
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 2
DP  - 2020 Feb 8
TI  - [Determination of four protease inhibitors in chicken by ultra performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 212-217
LID - 10.3724/SP.J.1123.2019.04047 [doi]
AB  - A method was developed for the determination of four protease inhibitors 
      (saquinavir, ritonavir, nelfinavir and indinavir) in chicken using ultra 
      performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The 
      samples were extracted by shaking with 30% (v/v) acetonitrile aqueous solution 
      (containing 1% (v/v) trichloroacetic acid), and purified by using mixed-mode 
      cationic-exchanger (MCX) cartridges. The samples were separated on a Luna((R)) C8 
      column (150 mmx2 mm, 3 mum) using 0.2% (v/v) formic acid aqueous solution 
      (containing 5 mmol/L ammonium acetate) and acetonitrile as the mobile phases with 
      gradient elution. The determination was carried out by using an electrospray ion 
      source in the positive and multiple-reaction monitoring (MRM) modes. The 
      calibration curves showed good linearities in the range of 0.1-20.0 mug/L, and the 
      correlation coefficients (r(2)) were greater than 0.99. The limits of 
      quantification (LOQs, S/N=10) of the four protease inhibitors varied from 0.20 
      mug/kg to 0.90 mug/kg. At the spiked levels of 1.0, 2.0, and 10.0 mug/kg, the 
      average recoveries of the four protease inhibitors were ranging from 69.0% to 
      106.0%. The intra-day and inter-day relative standard deviations (RSDs) were 
      2.2%-13.8% (n=6) and 3.6%-14.6% (n=3), respectively. The method is simple, 
      efficient, sensitive and accurate, and it can be used to detect residues of 
      saquinavir, ritonavir, nelfinavir and indinavir in chicken.
FAU - Lu, Jiale
AU  - Lu J
AD  - Food Science College of Fujian Agriculture and Forestry University, Fuzhou 
      350002, China.
FAU - Liu, Zhengcai
AU  - Liu Z
AD  - Technology center of Fuzhou Customs District, Fuzhou 350001, China.
FAU - Yao, Minna
AU  - Yao M
AD  - Food Science College of Fujian Agriculture and Forestry University, Fuzhou 
      350002, China.
FAU - Lin, Yuandi
AU  - Lin Y
AD  - Technology center of Fuzhou Customs District, Fuzhou 350001, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Protease Inhibitors)
RN  - 5W6YA9PKKH (Indinavir)
RN  - HO3OGH5D7I (Nelfinavir)
RN  - L3JE09KZ2F (Saquinavir)
RN  - O3J8G9O825 (Ritonavir)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chromatography, High Pressure Liquid
MH  - *Food Analysis
MH  - Food Contamination/*analysis
MH  - Indinavir
MH  - Nelfinavir
MH  - Poultry Products/*analysis
MH  - Protease Inhibitors/*analysis
MH  - Ritonavir
MH  - Saquinavir
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - chicken
OT  - indinavir
OT  - nelfinavir
OT  - ritonavir
OT  - saquinavir
OT  - solid phase extraction (SPE)
OT  - ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
EDAT- 2020/02/08 00:00
MHDA- 2021/07/17 06:00
CRDT- 2021/07/02 09:12
PHST- 2021/07/02 09:12 [entrez]
PHST- 2020/02/08 00:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.04047 [doi]
PST - ppublish
SO  - Se Pu. 2020 Feb 8;38(2):212-217. doi: 10.3724/SP.J.1123.2019.04047.