PMID- 34213185
OWN - NLM
STAT- MEDLINE
DCOM- 20210726
LR  - 20210726
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 8
DP  - 2020 Aug 8
TI  - [Combination of dispersive liquid-liquid microextraction using multifunctional 
      ionic liquids with high performance chromatography for determination of phthalate 
      ester metabolites in human urine sample].
PG  - 929-936
LID - 10.3724/SP.J.1123.2019.09026 [doi]
AB  - A novel sensitive method for the determination of the five primary metabolites of 
      phthalate esters (PAEs) in urine was developed by combining dispersive 
      liquid-liquid microextraction (DLLME) using ionic liquids with high performance 
      liquid chromatography (HPLC). The factors affecting the efficiency of DLLME were 
      optimized. The types and proportions of extraction solvent and dispersants, as 
      well the ultrasonic extraction time, cooling time, and centrifugal time, were 
      determined. The optimal conditions were as follows:extraction 
      solvent[C(8)MIM]PF(6)] 35 muL; dispersants[BSO(3)HMIm]OTf] 30 muL,[C(4)MIM]BF(6)] 
      120 muL; NH(4)PF(6) 0.1 g, extraction at 35℃, ultrasonic dispersion for 5 min, 
      cooling in ice water for 5 min, centrifugation at 4000 r/min for 5 min. After 
      optimization, the five primary metabolites of PAEs were determined. The method 
      showed a good linear relationship within the concentration range of 0.5-1000 
      mug/L. The determination coefficients (R(2)) were greater than 0.9955. The 
      detection limit was in the range of 0.16-0.19 mug/L. Under the optimized 
      conditions, the extraction recoveries for the PAEs were 92.9%-105.0%, and the 
      relative standard deviations (RSDs) of the intra- and inter-day precisions were < 
      5.96%. Urine samples collected from 10 diabetic patients were tested, and the 
      exposure level of the population to the PAE metabolites was evaluated. All the 
      PAE metabolites were detected in these samples, and the detection rate of 
      2-ethylhexyl hydrogen phthalate (MEHP) was 100%. In conclusion, no toxic organic 
      reagents were added during the extraction process in this method, and 
      multifunctional ionic liquids were used as the extraction agent, dispersant, and 
      salting-out agent. In other words, the extraction process was demonstrated to be 
      green, simple, and efficient. The developed method has high sensitivity and 
      stability, and it is suitable for the determination of trace PAE metabolites in 
      human urine.
FAU - Sun, Qian
AU  - Sun Q
AD  - Clinical Laboratory, Lihuili Hospital, Ningbo Municipal Medical Center, Ningbo 
      315040, China.
AD  - Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Medical 
      Genetics, Wenzhou 325035, China.
FAU - Dai, Haoqiang
AU  - Dai H
AD  - Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Medical 
      Genetics, Wenzhou 325035, China.
FAU - Chen, Peipei
AU  - Chen P
AD  - Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Medical 
      Genetics, Wenzhou 325035, China.
FAU - She, Hui
AU  - She H
AD  - Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Medical 
      Genetics, Wenzhou 325035, China.
FAU - Wu, Jia
AU  - Wu J
AD  - Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Medical 
      Genetics, Wenzhou 325035, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Esters)
RN  - 0 (Ionic Liquids)
RN  - 0 (Phthalic Acids)
RN  - 6O7F7IX66E (phthalic acid)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Esters/*urine
MH  - Humans
MH  - *Ionic Liquids
MH  - Limit of Detection
MH  - *Liquid Phase Microextraction
MH  - Phthalic Acids/*urine
OTO - NOTNLM
OT  - dispersive liquid-liquid microextraction (DLLME)
OT  - high performance liquid chromatography (HPLC)
OT  - multiple functional ionic liquids
OT  - phthalate metabolites
OT  - urine
EDAT- 2021/07/03 06:00
MHDA- 2021/07/27 06:00
CRDT- 2021/07/02 09:12
PHST- 2021/07/02 09:12 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/27 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.09026 [doi]
PST - ppublish
SO  - Se Pu. 2020 Aug 8;38(8):929-936. doi: 10.3724/SP.J.1123.2019.09026.