PMID- 34213204
OWN - NLM
STAT- MEDLINE
DCOM- 20210726
LR  - 20210726
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 6
DP  - 2020 Jun 8
TI  - [Determination of polycyclic aromatic hydrocarbon metabolites in urine by 
      liquid-liquid extraction-high resolution gas chromatography-high resolution 
      dual-focus magnetic mass spectrometry].
PG  - 715-721
LID - 10.3724/SP.J.1123.2019.10031 [doi]
AB  - A sensitive and accurate method was developed to quantify eight polycyclic 
      aromatic hydrocarbon (PAH) metabolites in human urine by liquid-liquid 
      extraction-high resolution gas chromatography-high resolution dual-focus magnetic 
      mass spectrometry (LLC-HRGC-HRMS). About 2 mL urine was mixed with a deuterium- 
      or (13)C-labeled isotopic internal standard, and the conjugated targets were 
      enzymatically digested in the presence of ascorbic acid. The free compounds were 
      extracted with toluene-pentane (1:4, v/v) and condensed to near dryness. Then, 
      the target compounds were redissolved in toluene. After derivatization, they were 
      separated and quantified by HRGC-HRMS. The linear ranges of the 
      1-hydroxynaphthalene, 1-hydroxyphenanthrene, and 2-hydroxyphenanthrene were 
      0.14-41.6 mug/L, 0.05-8.33 mug/L, and 0.04-8.33 mug/L, respectively, and those for 
      the other five PAH metabolites were 0.02-8.33 mug/L. The correlation coefficients 
      were greater than 0.99. The limits of detection were in the range of 0.006-0.042 
      mug/L, and the recoveries were 81.4%-127.0%. The intra-day and inter-day relative 
      standard deviations (RSDs) were less than 6.9% and 10.9%, respectively. This 
      method was utilized for the determination of 330 human urine samples. The results 
      showed that 3-hydroxychrysene and 6-hydroxychrysene were not detected, and the 
      detection rate of the other six PAH metabolites was 100%. This method is 
      sensitive, accurate and stable, and it is suitable for the determination of the 
      eight PAH metabolites in human urine.
FAU - Fu, Hui
AU  - Fu H
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
FAU - Lu, Yifu
AU  - Lu Y
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
FAU - Hu, Xiaojian
AU  - Hu X
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
FAU - Zhang, Xu
AU  - Zhang X
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
FAU - Yang, Yanwei
AU  - Yang Y
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
FAU - Zhu, Ying
AU  - Zhu Y
AD  - National Institute of Environmental Health, Chinese Center for Disease Control 
      and Prevention, Beijing 100021, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
SB  - IM
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Liquid-Liquid Extraction
MH  - Magnetic Phenomena
MH  - Polycyclic Aromatic Hydrocarbons/*urine
OTO - NOTNLM
OT  - biological monitoring
OT  - high resolution gas chromatography-high resolution dual-focus magnetic mass 
      spectrometry (HRGC-HRMS)
OT  - hydroxyl polycyclic aromatic metabolites
OT  - liquid-liquid extraction (LLE)
OT  - urine
EDAT- 2021/07/03 06:00
MHDA- 2021/07/27 06:00
CRDT- 2021/07/02 09:12
PHST- 2021/07/02 09:12 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/27 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.10031 [doi]
PST - ppublish
SO  - Se Pu. 2020 Jun 8;38(6):715-721. doi: 10.3724/SP.J.1123.2019.10031.