PMID- 34213239
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 5
DP  - 2020 May 8
TI  - [Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase 
      extraction-ultra performance liquid chromatography].
PG  - 547-553
LID - 10.3724/SP.J.1123.2019.09003 [doi]
AB  - A new method based on solid-phase extraction-ultraperformance liquid 
      chromatography (SPE-UPLC) was developed for the determination of five 
      protopanaxadiol ginsenosides in ginseng. The ginsenosides were extracted from 
      ground ginseng samples using water-saturated n-butanol and purified on a 
      hydrophilic solid-phase extraction column. Chromatographic separation was 
      achieved on an ACQUITY UPLC BEH Shield RP18 column (100 mmx2.1 mm, 1.7 mum) by 
      linear gradient elution using an acetonitrile/water mobile phase. Five 
      protopanaxadiol ginsenosides were detected by a photodiode array detector, and 
      they showed a strong positive linear correlation (r(2)>0.999) in the range of 
      5-500 mug/mL. In addition, the instrument precision ranged between 0.95% and 2.62% 
      (n=6), with the sample stability between 0.90% and 2.15% (n=8) within 22 h. 
      Intra- and inter-day repeatabilities were 5.35%-6.47% (n=6) and 5.56%-6.34% 
      (n=8), respectively. Sample recoveries and the corresponding relative standard 
      deviations (RSDs) were 87.16%-101.92% and 1.54%-4.01% (n=6), respectively. 
      Hydrophilic chromatography materials were used in SPE, and the extract was 
      directly loaded and purified without pretreatment. Besides, with the use of UPLC, 
      the analysis time was greatly shortened. The developed method is simple and 
      rapid, with high throughput, thus being suitable for the quantitative analysis of 
      the five protopanaxadiol ginsenosides in ginsengs.
FAU - Song, Chunying
AU  - Song C
AD  - Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Zhang, Huarong
AU  - Zhang H
AD  - DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China.
FAU - Guo, Zhimou
AU  - Guo Z
AD  - Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
AD  - DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China.
FAU - Yan, Jingyu
AU  - Yan J
AD  - Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
AD  - DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China.
FAU - Jin, Gaowa
AU  - Jin G
AD  - Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
AD  - DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China.
FAU - Liang, Xinmiao
AU  - Liang X
AD  - Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of 
      Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
AD  - DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Ginsenosides)
RN  - 0 (Sapogenins)
RN  - P6717R7BP8 (protopanaxadiol)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Ginsenosides/analysis
MH  - *Panax/chemistry
MH  - Sapogenins/*analysis
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - ginseng
OT  - ginsenoside
OT  - hydrophilic interaction chromatography (HILIC)
OT  - solid-phase extraction (SPE)
OT  - ultraperformance liquid chromatography (UPLC)
EDAT- 2020/05/08 00:00
MHDA- 2021/07/17 06:00
CRDT- 2021/07/02 09:13
PHST- 2021/07/02 09:13 [entrez]
PHST- 2020/05/08 00:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.09003 [doi]
PST - ppublish
SO  - Se Pu. 2020 May 8;38(5):547-553. doi: 10.3724/SP.J.1123.2019.09003.