PMID- 34213275
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 9
DP  - 2020 Sep 8
TI  - [Application of mixed polymer brushes based on poly(2-methyl-2-oxazoline) and 
      poly(acrylic acid) to on-line preconcentration of lysozyme by capillary 
      electrophoresis].
PG  - 1085-1094
LID - 10.3724/SP.J.1123.2020.02027 [doi]
AB  - A capillary coated with mixed polymer brushes that shows switchability toward 
      lysozyme adsorption was developed. This capillary was applied for the on-line 
      preconcentration of lysozyme by capillary electrophoresis (CE) in order to 
      enhance the detection sensitivity. First, poly(2-methyl-2-oxazoline) (PMOXA) and 
      poly(acrylic acid) (PAA) were synthesized by cationic ring-opening polymerization 
      and reversible addition-fragmentation chain transfer (RAFT) polymerization, 
      respectively. Then, glycidyl methacrylate (GMA) and PMOXA were used to prepare 
      poly(2-methyl-2-oxazoline)-random-glycidyl methacrylate (PMOXA-r-GMA) via radical 
      copolymerization, and poly(acrylic acid)-block-poly(glycidyl methacrylate) 
      (PAA-b-PGMA) was obtained by the RAFT polymerization of GMA and PAA. A mixed 
      solution of PMOXA-r-GMA and PAA-b-PGMA at a certain mass ratio was then injected 
      into the capillary. Subsequent annealing provided capillary materials coated with 
      mixed polymer brushes based on PMOXA and PAA. X-ray photoelectron spectroscopy 
      (XPS) analysis was performed to determine the surface composition of the 
      capillary raw materials. When the mass concentration of the mixed solution was 20 
      g/L and the mass ratio of PMOXA-r-GMA and PAA-b-PGMA was 1:1, the carboxyl 
      content in the coating increased with increasing chain length of PAA. Fluorescein 
      isothiocyanate-labeled lysozyme (FITC-lysozyme) adsorption assay demonstrated 
      that the coated capillary had switchable properties for lysozyme adsorption upon 
      pH and ionic strength (I) trigger. At pH 7 (I=10(-5)mol/L), the capillary could 
      adsorb a large amount of lysozyme, which could be released at pH 3 
      (I=10(-1)mol/L). Subsequently, this coated capillary was applied to the on-line 
      preconcentration of lysozyme by CE. The sensitivity enhancement factor was 17.69 
      when the chain length of PAA was 2.2 times that of PMOXA. The limit of detection 
      could reach 8.7x10(-5)g/L. On-line preconcentration of lysozyme was performed for 
      five successive times on the same day and on five consecutive days. The intraday 
      and interday relative standard deviations (RSDs) for the peak areas were 2.9% and 
      4.1%, respectively. The intraday and interday RSDs for the migration times were 
      0.9% and 2.1%, respectively. The developed method for the preparation of the 
      coated capillary with good stability only needs one step in this work, and this 
      research will supply a simple and effective way to analyze trace protein by CE.
FAU - Zhang, Miao
AU  - Zhang M
AD  - Department of Polymer Science and Engineering, University of Science and 
      Technology of China, Hefei 230026, China.
FAU - Wang, Yuchen
AU  - Wang Y
AD  - Department of Polymer Science and Engineering, University of Science and 
      Technology of China, Hefei 230026, China.
FAU - Muhammad, Atif
AU  - Muhammad A
AD  - Department of Polymer Science and Engineering, University of Science and 
      Technology of China, Hefei 230026, China.
FAU - Chen, Lijuan
AU  - Chen L
AD  - College of Materials and Chemical Engineering, West Anhui University, Lu'an 
      237012, China.
FAU - Wang, Yanmei
AU  - Wang Y
AD  - Department of Polymer Science and Engineering, University of Science and 
      Technology of China, Hefei 230026, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acrylic Resins)
RN  - 0 (Polyamines)
RN  - 0 (Polymers)
RN  - 0 (poly(2-methyl-2-oxazoline))
RN  - 4Q93RCW27E (carbopol 940)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Acrylic Resins
MH  - *Electrophoresis, Capillary
MH  - *Muramidase
MH  - *Polyamines
MH  - *Polymers
OTO - NOTNLM
OT  - capillary electrophoresis (CE)
OT  - mixed polymer brushes
OT  - on-line preconcentration
OT  - poly(2-methyl-2-oxazoline) (PMOXA)
OT  - poly(acrylic acid) (PAA)
EDAT- 2021/07/03 06:00
MHDA- 2021/07/17 06:00
CRDT- 2021/07/02 09:13
PHST- 2021/07/02 09:13 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
AID - 10.3724/SP.J.1123.2020.02027 [doi]
PST - ppublish
SO  - Se Pu. 2020 Sep 8;38(9):1085-1094. doi: 10.3724/SP.J.1123.2020.02027.