PMID- 34213289
OWN - NLM
STAT- MEDLINE
DCOM- 20210726
LR  - 20210726
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 38
IP  - 7
DP  - 2020 Jul 8
TI  - [Simultaneous determination of 37 mycotoxins in grain and animal feed by impurity 
      adsorption purification coupled with ultra-performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 817-825
LID - 10.3724/SP.J.1123.2019.12013 [doi]
AB  - A rapid method based on ultra-performance liquid chromatography-tandem mass 
      spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 37 
      mycotoxins having various physicochemical properties in grain and animal feed 
      samples. The 37 analytes were extracted from ground samples with 
      acetonitrile-water-formic acid (84:15.9:0.1, v/v/v) by 20 min vibration, and 
      purified using a commercial MLJ-1 pass-through solid-phase extraction (SPE) 
      clean-up cartridge. The analytes were then separated on a reversed-phase BEH RP18 
      column by a gradient elution program with 0.1 mmol/L ammonium acetate (containing 
      0.1% (v/v) formic acid) aqueous solution and 0.1% (v/v) formic acid methanol 
      solution as mobile phases. The separated analytes were detected by MS/MS in the 
      multiple reaction monitoring (MRM) mode via ESI+/- ionization. The results showed 
      that the purification was completed in 1 min and that the 37 analytes could be 
      separated on the chromatographic column in 15 min. The 37 mycotoxins showed a 
      linear relationship within their respective linear ranges, and the correlation 
      coefficients of the matrix-matched calibration curves were greater than 0.98. The 
      average recoveries at four spiked levels (limit of quantification (LOQ), LOQx5, 
      LOQx10, LOQx25) for all the targets except fumonisins ranged from 80% to 120%, 
      with the relative standard deviations (RSDs) lower than 20% (n=6). The limits of 
      quantification (LOQs) for all the analytes were between 2 and 40 mug/kg. The 
      proposed method is simple, fast, and accurate, thus being suitable for detecting 
      multiple mycotoxins in grain and animal feed samples.
FAU - Wang, Ruiguo
AU  - Wang R
AD  - Institute of Quality Standards and Testing Technology for Agricultural Products, 
      Chinese Academy of Agricultural Science, Key Laboratory of Agrifood Safety and 
      Quality, Ministry of Agriculture, Beijing 100081, China.
FAU - Guo, Lili
AU  - Guo L
AD  - Institute of Quality Standards and Testing Technology for Agricultural Products, 
      Chinese Academy of Agricultural Science, Key Laboratory of Agrifood Safety and 
      Quality, Ministry of Agriculture, Beijing 100081, China.
FAU - Wang, Peilong
AU  - Wang P
AD  - Institute of Quality Standards and Testing Technology for Agricultural Products, 
      Chinese Academy of Agricultural Science, Key Laboratory of Agrifood Safety and 
      Quality, Ministry of Agriculture, Beijing 100081, China.
FAU - Su, Xiao'ou
AU  - Su X
AD  - Institute of Quality Standards and Testing Technology for Agricultural Products, 
      Chinese Academy of Agricultural Science, Key Laboratory of Agrifood Safety and 
      Quality, Ministry of Agriculture, Beijing 100081, China.
FAU - Song, Zhichao
AU  - Song Z
AD  - Henan Institute of Veterinary Drug and Feed Control, Zhengzhou 450008, China.
FAU - Lin, Gang
AU  - Lin G
AD  - Alltech Biological Products(China)Co., Ltd, Beijing 100600, China.
FAU - Zhu, Ronghua
AU  - Zhu R
AD  - Alltech Biological Products(China)Co., Ltd, Beijing 100600, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Adsorption
MH  - Animal Feed/*analysis
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Edible Grain/*anatomy & histology
MH  - Food Analysis/*methods
MH  - Food Contamination/*analysis
MH  - *Mycotoxins/analysis
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - feed
OT  - grain
OT  - impurity adsorption
OT  - mycotoxins
OT  - solid phase extraction (SPE)
OT  - ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
EDAT- 2021/07/03 06:00
MHDA- 2021/07/27 06:00
CRDT- 2021/07/02 09:13
PHST- 2021/07/02 09:13 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/27 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.12013 [doi]
PST - ppublish
SO  - Se Pu. 2020 Jul 8;38(7):817-825. doi: 10.3724/SP.J.1123.2019.12013.