PMID- 34213992
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20221207
IS  - 1520-5762 (Electronic)
IS  - 0363-9045 (Linking)
VI  - 47
IP  - 5
DP  - 2021 May
TI  - Establishment of quantitative methodology for sophoridine analysis and 
      determination of its pharmacokinetics and bioavailability in rat.
PG  - 741-747
LID - 10.1080/03639045.2021.1934862 [doi]
AB  - OBJECTIVE: The aim of this study is to develop a rapid and sensitive UPLC-MS/MS 
      approach to determine the sophoridine (SOP) level in rat plasma and the 
      pharmacokinetics of the substance. SIGNIFICANCE: Sophoridine is used as an 
      anti-inflammatory, anti-virus, anti-microbial, and anti-tumor alkaloid. It is 
      essential to explore specific detection methods for the quantitative analysis of 
      SOP in the blood circulation. METHODS: The rat plasma samples were prepared by 
      one-step protein precipitation with acetonitrile. Subsequently, the samples were 
      separated by chromatography using a UPLC BEH C18 reversed-phase with an initial 
      mobile phase of methanol and 0.1% formic acid aqueous solution. The gradient 
      elution was performed at a fixed flow rate of 0.4 mL/min, and multiple reaction 
      monitoring (MRM) mode with an electrospray positive ionization source was 
      employed to detect the transitions of m/z 249.1 --> 84.2 for SOP and m/z 264.3 --> 
      69.8 for dendrobine (IS). The entire process required 3.5 min for each sample. 
      RESULTS: A linear correlation was established over the range of 2-2000 ng/mL 
      (r(2)>/=0.9954) for SOP in rat plasma with a lower limit of quantification (LLOQ) 
      at 2 ng/mL. The range of accuracy was tested between 94.90% and 100.80%, and the 
      relative standard deviations (RSDs) toward both intra- and inter-day precision 
      were <10%. Thus, this method was successfully applied to a pharmacokinetic study, 
      and the subsequent results demonstrated a low absolute bioavailability of 2.32%. 
      CONCLUSION: The present study established a reliable method that quantified the 
      SOP concentration in rat plasma after administering a dose of 2 mg/kg 
      intravenously or 20 mg/kg orally.
FAU - Chen, Mengchun
AU  - Chen M
AD  - Department of Pharmacy, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, Wenzhou, China.
AD  - Wenzhou Municipal Key Laboratory of Pediatric Pharmacy, Wenzhou, China.
FAU - Jiang, Qi
AU  - Jiang Q
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Zhang, Mingyao
AU  - Zhang M
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Chen, Sailing
AU  - Chen S
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Lou, Junsheng
AU  - Lou J
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
AD  - Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Chen, Yijie
AU  - Chen Y
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Wang, Fang
AU  - Wang F
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Wang, Rongyue
AU  - Wang R
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Drug Dev Ind Pharm
JT  - Drug development and industrial pharmacy
JID - 7802620
RN  - 0 (Alkaloids)
RN  - 0 (Quinolizines)
RN  - 0 (Matrines)
SB  - IM
MH  - *Alkaloids
MH  - Animals
MH  - Biological Availability
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Quinolizines
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - *Tandem Mass Spectrometry
MH  - Matrines
OTO - NOTNLM
OT  - Sophoridine
OT  - UPLC-MS/MS
OT  - bioavailability
OT  - pharmacokinetics
OT  - rat plasma
EDAT- 2021/07/03 06:00
MHDA- 2021/07/07 06:00
CRDT- 2021/07/02 17:12
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
PHST- 2021/07/02 17:12 [entrez]
AID - 10.1080/03639045.2021.1934862 [doi]
PST - ppublish
SO  - Drug Dev Ind Pharm. 2021 May;47(5):741-747. doi: 10.1080/03639045.2021.1934862.