PMID- 34216240
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20210721
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 78
IP  - 8
DP  - 2021 Aug
TI  - Halophilic Prokaryotes in Urmia Salt Lake, a Hypersaline Environment in Iran.
PG  - 3230-3238
LID - 10.1007/s00284-021-02583-w [doi]
AB  - In this study, fluorescence in situ hybridization (FISH) and PCR-amplified 
      fragments of the 16SrDNA gene were used to determine prokaryotes diversity in 
      Urmia Salt Lake. Prokaryote cell population in Urmia lake range from 
      3.1 +/- 0.3 x 10(6), 2 +/- 0.2 x 10(8), 4 +/- 0.3 x 10(8), and 1.8 +/- 0.2 x 10(8) cells 
      ml(-1) for water, soil, sediment, and salt samples by DAPI (4́, 
      6-diamidino-2-phenylindole) direct count, respectively. The proportion of 
      bacteria and archaea in the samples determinable by FISH ranged between 36.1 and 
      55% and 48.5 and 55.5%, respectively. According to the DGGE method, some bands 
      were selected and separated from the gel, then amplified and sequenced. The 
      results of sequences were related to two phyla Proteobacteria (16.6%) and 
      Bacteroidetes (83.3%), which belonged to four genera Salinibacter, 
      Mangroviflexus, Pseudomonas, and Cesiribacter, and the archaeal sequences were 
      related to Euryarchaeota phyla and three genera Halonotius, Haloquadratum, and 
      Halorubrum. According to our results, it seems that prokaryotic populations in 
      this hypersaline environment are more diverse than expected, and bacteria are so 
      abundant and diverse and form the metabolically active part of the microbial 
      population inhabiting this extreme environment. Molecular dependent and 
      independent approaches revealed a different aspect of this environment 
      microbiota.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Jookar Kashi, Fereshteh
AU  - Jookar Kashi F
AD  - University of Kashan, Kashan, Iran. jookar@kashanu.ac.ir.
FAU - Owlia, Parviz
AU  - Owlia P
AD  - University of Kashan, Kashan, Iran.
FAU - Amoozegar, Mohammad Ali
AU  - Amoozegar MA
AD  - University of Kashan, Kashan, Iran.
FAU - Kazemi, Bahram
AU  - Kazemi B
AD  - University of Kashan, Kashan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210703
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
EIN - Curr Microbiol. 2021 Sep;78(9):3596. PMID: 34287661
MH  - *Archaea/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Iran
MH  - *Lakes
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Water Microbiology
EDAT- 2021/07/04 06:00
MHDA- 2021/07/22 06:00
CRDT- 2021/07/03 12:07
PHST- 2020/08/31 00:00 [received]
PHST- 2021/04/14 00:00 [accepted]
PHST- 2021/07/04 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2021/07/03 12:07 [entrez]
AID - 10.1007/s00284-021-02583-w [pii]
AID - 10.1007/s00284-021-02583-w [doi]
PST - ppublish
SO  - Curr Microbiol. 2021 Aug;78(8):3230-3238. doi: 10.1007/s00284-021-02583-w. Epub 
      2021 Jul 3.