PMID- 34216677
OWN - NLM
STAT- MEDLINE
DCOM- 20210901
LR  - 20210901
IS  - 1872-9428 (Electronic)
IS  - 0166-6851 (Linking)
VI  - 244
DP  - 2021 Jul
TI  - A store-operated Ca(2+)-entry in Trypanosoma equiperdum: Physiological evidences 
      of its presence.
PG  - 111394
LID - S0166-6851(21)00041-4 [pii]
LID - 10.1016/j.molbiopara.2021.111394 [doi]
AB  - The Trypanosomatidae family encompasses many unicellular organisms responsible of 
      several tropical diseases that affect humans and animals. Livestock 
      tripanosomosis caused by Trypanosoma brucei brucei (T. brucei), Trypanosoma 
      equiperdum (T. equiperdum) and Trypanosoma evansi (T. evansi), have a significant 
      socio-economic impact and limit animal protein productivity throughout the 
      intertropical zones of the world. Similarly, to all organisms, the maintenance of 
      Ca(2+) homeostasis is vital for these parasites, and the mechanism involved in 
      the intracellular Ca(2+) regulation have been widely described. However, the 
      evidences related to the mechanisms responsible for the Ca(2+) entry are scarce. 
      Even more, to date the presence of a store-operated Ca(2+) channel (SOC) has not 
      been reported. Despite the apparent absence of Orai and STIM-like proteins in 
      these parasites, in the present work we demonstrate the presence of a 
      store-operated Ca(2+)-entry (SOCE) in T. equiperdum, using physiological 
      techniques. This Ca(2+)-entry is induced by thapsigargin (TG) and 
      2,5-di-t-butyl-1,4-benzohydroquinone (BHQ), and inhibited by 
      2-aminoethoxydiphenyl borate (2APB). Additionally, the use of bioinformatics 
      techniques allowed us to identify putative transient receptor potential (TRP) 
      channels, present in members of the Trypanozoon family, which would be possible 
      candidates responsible for the SOCE described in the present work in T. 
      equiperdum.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Perez-Gordones, Maria C
AU  - Perez-Gordones MC
AD  - Instituto de Biologia Experimental (IBE), Universidad Central de Venezuela (UCV), 
      Caracas, Venezuela.
FAU - Ramirez-Iglesias, Jose R
AU  - Ramirez-Iglesias JR
AD  - Group of Neglected and Emerging Diseases, Epidemiology and Biodiversity, Health 
      Sciences Faculty, Universidad Internacional SEK (UISEK), Quito, Ecuador.
FAU - Benaim, Gustavo
AU  - Benaim G
AD  - Instituto de Biologia Experimental (IBE), Universidad Central de Venezuela (UCV), 
      Caracas, Venezuela; Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela.
FAU - Mendoza, Marta
AU  - Mendoza M
AD  - Centro de Estudios Biomedicos y Veterinarios, Instituto de Estudios Cientificos y 
      Tecnologicos (IDECYT), Universidad Nacional Experimental Simon Rodriguez, 
      Caracas, Venezuela. Electronic address: mendozamarta17@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210701
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Boron Compounds)
RN  - 0 (Calcium Chelating Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Hydroquinones)
RN  - 0 (Intracellular Calcium-Sensing Proteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 67526-95-8 (Thapsigargin)
RN  - E4ES684O93 (2-aminoethoxydiphenyl borate)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Boron Compounds/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Chelating Agents/chemistry
MH  - Computational Biology/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes/chemistry
MH  - Fura-2/chemistry
MH  - Gene Expression
MH  - Homeostasis/genetics
MH  - Hydroquinones/pharmacology
MH  - Intracellular Calcium-Sensing Proteins/genetics/*metabolism
MH  - Manganese/metabolism
MH  - Protozoan Proteins/genetics/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Transient Receptor Potential Channels/genetics/*metabolism
MH  - Trypanosoma/drug effects/genetics/*metabolism
MH  - Trypanosomiasis/parasitology
OTO - NOTNLM
OT  - 2, 5-di-t-butyl-1, 4-benzohydroquinone (BHQ)
OT  - 2-aminoethoxydiphenyl borate (2APB)
OT  - Ca(2+)-channel
OT  - Store operated calcium entry (SOCE)
OT  - Thapsigargin (TG)
OT  - Trypanosoma equiperdum
EDAT- 2021/07/04 06:00
MHDA- 2021/09/02 06:00
CRDT- 2021/07/03 20:10
PHST- 2021/01/17 00:00 [received]
PHST- 2021/06/26 00:00 [revised]
PHST- 2021/06/28 00:00 [accepted]
PHST- 2021/07/04 06:00 [pubmed]
PHST- 2021/09/02 06:00 [medline]
PHST- 2021/07/03 20:10 [entrez]
AID - S0166-6851(21)00041-4 [pii]
AID - 10.1016/j.molbiopara.2021.111394 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 2021 Jul;244:111394. doi: 
      10.1016/j.molbiopara.2021.111394. Epub 2021 Jul 1.