PMID- 34217210
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20210708
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 22
IP  - 1
DP  - 2021 Jul 3
TI  - Identification of long noncoding RNAs reveals the effects of dinotefuran on the 
      brain in Apis mellifera (Hymenopptera: Apidae).
PG  - 502
LID - 10.1186/s12864-021-07811-y [doi]
LID - 502
AB  - BACKGROUND: Dinotefuran (CAS No. 165252-70-0), a neonicotinoid insecticide, has 
      been used to protect various crops against invertebrate pests and has been 
      associated with numerous negative sublethal effects on honey bees. Long noncoding 
      RNAs (lncRNAs) play important roles in mediating various biological and 
      pathological processes, involving transcriptional and gene regulation. The 
      effects of dinotefuran on lncRNA expression and lncRNA function in the honey bee 
      brain are still obscure. RESULTS: Through RNA sequencing, a comprehensive 
      analysis of lncRNAs and mRNAs was performed following exposure to 0.01 mg/L 
      dinotefuran for 1, 5, and 10 d. In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs 
      and 1458 mRNAs, and 345 lncRNAs and 1155 mRNAs were found to be differentially 
      expressed (DE) on days 1, 5 and 10, respectively. Gene set enrichment analysis 
      (GSEA) indicated that the dinotefuran-treated group showed enrichment in 
      carbohydrate and protein metabolism and immune-inflammatory responses such as 
      glycine, serine and threonine metabolism, pentose and glucuronate 
      interconversion, and Hippo and transforming growth factor-beta (TGF-beta) signaling 
      pathways. Moreover, the DE lncRNA TCONS_00086519 was shown by fluorescence in 
      situ hybridization (FISH) to be distributed mainly in the cytoplasm, suggesting 
      that it may serve as a competing endogenous RNA and a regulatory factor in the 
      immune response to dinotefuran. CONCLUSION: This study characterized the 
      expression profile of lncRNAs upon exposure to neonicotinoid insecticides in 
      young adult honey bees and provided a framework for further study of the role of 
      lncRNAs in honey bee growth and the immune response.
FAU - Huang, Minjie
AU  - Huang M
AD  - Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of 
      Agricultural Sciences, Hangzhou, China.
FAU - Dong, Jie
AU  - Dong J
AD  - Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of 
      Agricultural Sciences, Hangzhou, China.
FAU - Guo, Haikun
AU  - Guo H
AD  - Institute of Quality and Standard for Agro-Products, Zhejiang Academy of 
      Agricultural Sciences, Hangzhou, China.
FAU - Xiao, Minghui
AU  - Xiao M
AD  - Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of 
      Agricultural Sciences, Hangzhou, China.
AD  - State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, 
      Hangzhou, China.
FAU - Wang, Deqian
AU  - Wang D
AD  - Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of 
      Agricultural Sciences, Hangzhou, China. wangdq@zaas.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20210703
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Guanidines)
RN  - 0 (Neonicotinoids)
RN  - 0 (Nitro Compounds)
RN  - 0 (RNA, Long Noncoding)
RN  - 1W509710WF (dinotefuran)
SB  - IM
MH  - Animals
MH  - Bees
MH  - Brain
MH  - Guanidines
MH  - In Situ Hybridization, Fluorescence
MH  - Neonicotinoids
MH  - Nitro Compounds
MH  - *RNA, Long Noncoding
PMC - PMC8254963
OTO - NOTNLM
OT  - Apis mellifera
OT  - Brain
OT  - Dinotefuran
OT  - Neonicotinoid insecticide
OT  - lncRNA
COIS- The authors declare that they have no competing interests.
EDAT- 2021/07/05 06:00
MHDA- 2021/07/07 06:00
PMCR- 2021/07/03
CRDT- 2021/07/04 20:21
PHST- 2021/03/10 00:00 [received]
PHST- 2021/06/14 00:00 [accepted]
PHST- 2021/07/04 20:21 [entrez]
PHST- 2021/07/05 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
PHST- 2021/07/03 00:00 [pmc-release]
AID - 10.1186/s12864-021-07811-y [pii]
AID - 7811 [pii]
AID - 10.1186/s12864-021-07811-y [doi]
PST - epublish
SO  - BMC Genomics. 2021 Jul 3;22(1):502. doi: 10.1186/s12864-021-07811-y.