PMID- 34217974 OWN - NLM STAT- MEDLINE DCOM- 20211118 LR - 20211118 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 567 DP - 2021 Aug 27 TI - CCT5 interacts with cyclin D1 promoting lung adenocarcinoma cell migration and invasion. PG - 222-229 LID - S0006-291X(21)00725-7 [pii] LID - 10.1016/j.bbrc.2021.04.105 [doi] AB - Cyclin D1 (CCND1) has been identified as a metastatic promoter in various tumors including lung adenocarcinoma (LUAD), a subtype of non small cell lung cancer (NSCLC). The previous observation revealed that CCND1 was upregulated in NSCLC and predicted poor prognosis of LUAD patients. In this study, we examined a chaperonin containing TCP1 subunit 5 (CCT5) protein interacts with CCND1 in LUAD. Immunofluorescence demonstrated the co-localization of CCT5 and CCND1 protein in LUAD cells. CCT5 expression was detected with both immunohistochemistry (IHC) and bioinformatics analyses. Similar with the expression pattern of CCND1, CCT5 displayed a high level in LUAD tissues compared to non cancerous lung specimens. Patients with high CCT5 expression showed a significant shorter overall survival relative to those with low expression level. Furthermore, upregulated CCT5 exhibited significant positive correlation with TNM stage of LUAD patients in both IHC analyses and bioinformatics. Knocking down CCT5 remarkably inhibited LUAD cell migration and invasion in vitro by inactivating PI3K/AKT and its downstream EMT signals, which could abrogated the accelerated migration and invasion caused by CCND1 overexpression. In summary, our study discovered a highly expressed protein CCT5 in LUAD which interacted with CCND1 and promoted migration and invasion of LUAD cells by positively moderating PI3K/AKT-induced EMT pathway. CI - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Meng, Yiliang AU - Meng Y AD - Department of Oncology, Baise People's Hospital, Guangxi, Baise, 33000, Guangxi, China. FAU - Yang, Liu AU - Yang L AD - Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, 510095, PR China. FAU - Wei, Xiao AU - Wei X AD - Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, 510095, PR China. FAU - Luo, Hongcheng AU - Luo H AD - Department of Laboratory Medicine, PR China. FAU - Hu, Yingying AU - Hu Y AD - Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, 510095, PR China. FAU - Tao, Xingyu AU - Tao X AD - Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, 510095, PR China. FAU - He, Jingjing AU - He J AD - Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, 510095, PR China. FAU - Zheng, Xuesong AU - Zheng X AD - Department of Oncology, Baise People's Hospital, Guangxi, Baise, 33000, Guangxi, China. FAU - Xu, Qunying AU - Xu Q AD - Department of Oncology, Baise People's Hospital, Guangxi, Baise, 33000, Guangxi, China. FAU - Luo, Kunxiang AU - Luo K AD - Department of Neurosurgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi, BaiSe, 533000, China. FAU - Yu, Guifang AU - Yu G AD - Department of Oncology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. Electronic address: 526136010@qq.com. FAU - Luo, Qisheng AU - Luo Q AD - Department of Neurosurgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Guangxi, BaiSe, 533000, China. Electronic address: 850198155@qq.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210701 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (CCT5 protein, human) RN - 136601-57-5 (Cyclin D1) RN - EC 3.6.1.- (Chaperonin Containing TCP-1) SB - IM MH - Adenocarcinoma of Lung/*metabolism/pathology MH - Cell Line, Tumor MH - Cell Movement MH - Chaperonin Containing TCP-1/*metabolism MH - Cyclin D1/*metabolism MH - Humans MH - Lung Neoplasms/*metabolism/pathology MH - Neoplasm Invasiveness/pathology MH - Protein Interaction Maps OTO - NOTNLM OT - CCND1 OT - CCT5 OT - Invasion OT - LUAD OT - Migration OT - PI3K/AKT COIS- Declaration of competing interest We declare no conflict of interest. EDAT- 2021/07/05 06:00 MHDA- 2021/11/19 06:00 CRDT- 2021/07/04 20:47 PHST- 2021/04/19 00:00 [received] PHST- 2021/04/25 00:00 [accepted] PHST- 2021/07/05 06:00 [pubmed] PHST- 2021/11/19 06:00 [medline] PHST- 2021/07/04 20:47 [entrez] AID - S0006-291X(21)00725-7 [pii] AID - 10.1016/j.bbrc.2021.04.105 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2021 Aug 27;567:222-229. doi: 10.1016/j.bbrc.2021.04.105. Epub 2021 Jul 1.