PMID- 34219741
OWN - NLM
STAT- MEDLINE
DCOM- 20211230
LR  - 20211230
IS  - 0973-7138 (Electronic)
IS  - 0250-5991 (Linking)
VI  - 46
DP  - 2021
TI  - N-3-(oxododecanoyl)-L-homoserine lactone suppresses dendritic cell maturation by 
      upregulating the long noncoding RNA NRIR.
LID - 65 [pii]
AB  - N-3-(oxododecanoyl)-L-homoserine lactone (3-O-C12-HSL), a small bacterial 
      signaling molecule secreted by Pseudomonas aeruginosa (P. aeruginosa), can block 
      dendritic cell (DC) maturation and participate in immune escape, but the 
      underlying mechanism is unclear. We speculate that regulation of DC maturation 
      and function by lncRNAs may be the mechanism by which 3-O-C12-HSL inhibits the 
      immune response. We found that 3-O-C12-HSL increased the expression level of the 
      lncRNA NRIR, impeding monocyte-derived dendritic cell (Mo-DC) maturation. In 
      addition, we observed the effect of NRIR on the expression of CD40, CD80, HLA-DR 
      and IL-6. NRIR overexpression significantly reduced the expression of Mo-DC 
      surface markers, while 3-OC12-HSL did not significantly reduce the expression of 
      Mo-DC surface markers after NRIR knockdown. These results indicate that 
      3-O-C12-HSL indeed affects the differentiation and maturation of Mo-DCs through 
      NRIR. IL-6 stimulates T cell proliferation and activation, and we found that high 
      NRIR expression reduced IL6 levels. However, under NRIR knockdown, 3-O-C12-HSL 
      did not decrease IL-6 expression, suggesting that 3-O-C12-HSL may affect T cell 
      activation through NRIR. This study is the first to elucidate the important role 
      of a lncRNA in the mechanism of 3-O-C12-HSL activity. It also provides new ideas 
      regarding P. aeruginosa infection pathogenesis.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese 
      Medicine, Guangzhou 510006, People's Republic of China.
FAU - Liu, Yang
AU  - Liu Y
FAU - Lu, Yang
AU  - Lu Y
FAU - Li, Song
AU  - Li S
FAU - Liu, Jianping
AU  - Liu J
FAU - Zhang, Yunyan
AU  - Zhang Y
FAU - Wang, Lina
AU  - Wang L
FAU - Li, M O
AU  - Li MO
FAU - Luo, Yanfen
AU  - Luo Y
FAU - Zhang, Weizheng
AU  - Zhang W
FAU - Chen, Cha
AU  - Chen C
FAU - Li, Youqiang
AU  - Li Y
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Biosci
JT  - Journal of biosciences
JID - 8100809
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (N-(3-oxododecanoyl)homoserine lactone)
RN  - 0 (RNA, Long Noncoding)
RN  - 6KA95X0IVO (Homoserine)
RN  - OL659KIY4X (4-Butyrolactone)
SB  - IM
MH  - 4-Butyrolactone/*analogs & derivatives/pharmacology
MH  - Case-Control Studies
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/*drug effects
MH  - Homoserine/*analogs & derivatives/pharmacology
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Monocytes/metabolism
MH  - Pseudomonas Infections/metabolism
MH  - Pseudomonas aeruginosa
MH  - RNA, Long Noncoding/*metabolism
EDAT- 2021/07/06 06:00
MHDA- 2021/12/31 06:00
CRDT- 2021/07/05 09:49
PHST- 2021/07/05 09:49 [entrez]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/12/31 06:00 [medline]
AID - 65 [pii]
PST - ppublish
SO  - J Biosci. 2021;46:65.