PMID- 34220742
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210706
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 12
DP  - 2021
TI  - Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and 
      Activation of CD4(+) T Cells.
PG  - 653587
LID - 10.3389/fmicb.2021.653587 [doi]
LID - 653587
AB  - Exopolysaccharide (EPS) is a bacterial extracellular carbohydrate moiety which 
      has been associated with immunomodulatory activity and host protective effects of 
      several gut commensal bacteria. Bifidobacterium breve are early colonizers of the 
      human gastrointestinal tract (GIT) but the role of EPS in mediating their effects 
      on the host has not been investigated for many strains. Here, we characterized 
      EPS production by a panel of human B. breve isolates and investigated the effect 
      of EPS status on host immune responses using human and murine cell culture-based 
      assay systems. We report that B. breve EPS production is heterogenous across 
      strains and that immune responses in human THP-1 monocytes are strain-specific, 
      but not EPS status-specific. Using wild type and isogenic EPS deficient mutants 
      of B. breve strains UCC2003 and JCM7017 we show that EPS had strain-specific 
      divergent effects on cytokine responses from murine bone marrow derived 
      macrophages (BMDMs) and dendritic cells (BMDCs). The B. breve UCC2003 EPS 
      negative (EPS(-)) strain increased expression of cytokine genes (Tnfa, Il6, 
      Il12a, and Il23a) relative to untreated BMDCs and BMDCs treated with wild type 
      strain. B. breve UCC2003 and JCM7017 EPS(-) strains increased expression of 
      dendritic cell (DC) activation and maturation marker genes (Cd80, Cd83, and Cd86) 
      relative to untreated BMDCs. Consistent with this, BMDCs co-cultured with B. 
      breve UCC2003 and JCM7017 EPS(-) strains engineered to express OVA antigen 
      activated OVA-specific OT-II CD4(+) T-cells in a co-culture antigen-presentation 
      assay while EPS proficient strains did not. Collectively, these data indicate 
      that B. breve EPS proficient strains use EPS to prevent maturation of DCs and 
      activation of antigen specific CD4(+) T cells responses to B. breve. This study 
      identifies a new immunomodulatory role for B. breve EPS and suggests it may be 
      important for immune evasion of adaptive immunity by B. breve and contribute to 
      host-microbe mutualism.
CI  - Copyright (c) 2021 Hickey, Stamou, Udayan, Ramon-Vazquez, Esteban-Torres, 
      Bottacini, Woznicki, Hughes, Melgar, Ventura, Van Sinderen, Rossini and Nally.
FAU - Hickey, Ana
AU  - Hickey A
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
FAU - Stamou, Panagiota
AU  - Stamou P
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Udayan, Sreeram
AU  - Udayan S
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
FAU - Ramon-Vazquez, Ana
AU  - Ramon-Vazquez A
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Esteban-Torres, Maria
AU  - Esteban-Torres M
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Bottacini, Francesca
AU  - Bottacini F
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Woznicki, Jerzy Adam
AU  - Woznicki JA
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Hughes, Owen
AU  - Hughes O
AD  - Luminex Corporation, Austin, TX, United States.
FAU - Melgar, Silvia
AU  - Melgar S
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Ventura, Marco
AU  - Ventura M
AD  - Life Sciences and Environmental Sustainability, University of Parma, Parma, 
      Italy.
FAU - Van Sinderen, Douwe
AU  - Van Sinderen D
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Rossini, Valerio
AU  - Rossini V
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Nally, Ken
AU  - Nally K
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
LA  - eng
PT  - Journal Article
DEP - 20210616
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8242212
OTO - NOTNLM
OT  - Bifidobacterium breve
OT  - antigen presentation
OT  - dendritic cells
OT  - exopolysaccharides
OT  - host immune responses
OT  - macrophages
COIS- OH is employed by the company Luminex Corporation which manufactures the Amnis 
      ImageStream flow cytometer used in this study. The remaining authors declare that 
      the research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2021/07/06 06:00
MHDA- 2021/07/06 06:01
PMCR- 2021/06/16
CRDT- 2021/07/05 10:00
PHST- 2021/01/14 00:00 [received]
PHST- 2021/05/11 00:00 [accepted]
PHST- 2021/07/05 10:00 [entrez]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/07/06 06:01 [medline]
PHST- 2021/06/16 00:00 [pmc-release]
AID - 10.3389/fmicb.2021.653587 [doi]
PST - epublish
SO  - Front Microbiol. 2021 Jun 16;12:653587. doi: 10.3389/fmicb.2021.653587. 
      eCollection 2021.