PMID- 34221103
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 0974-5963 (Print)
IS  - 1742-6413 (Electronic)
IS  - 1742-6413 (Linking)
VI  - 18
DP  - 2021
TI  - A comparative study for qualitative and quantitative analysis of light and 
      fluorescence microscopy stains in oral premalignant and malignant lesions.
PG  - 14
LID - 10.25259/Cytojournal_39_2020 [doi]
LID - 14
AB  - OBJECTIVES: The present study highlights the use and benefit of fluorescence 
      microscopy stains in easy diagnosis of oral premalignant and malignant lesions. 
      This study was carried out to evaluate the efficiency of light microscopy using 
      Papanicolaou stain (PAP) and May Grunwald Giemsa (MGG) stains and fluorescence 
      microscopy using acridine orange (AO) and 4',6-diamidino-2-phenyl indole (DAPI) 
      stains. All smears have been qualitatively and quantitatively analyzed in 
      premalignant and malignant lesions using cytology and cytomorphometry. MATERIALS 
      AND METHODS: The subjects included in this study were selected from patients 
      suffering from premalignant and clinically suspicious malignant lesions. 
      Specimens were collected from the most representative site in study groups and 
      normal buccal mucosa in the control group. The smears were evaluated under 
      fluorescence microscopy for AO and DAPI and under light microscopy for 
      conventional PAP and MGG. We studied 5 HPF to compare the number of normal cells 
      to dysplastic cells. These were then subjected for cytomorphometric and 
      statistical analysis. RESULTS: Assessment of quality using quality index revealed 
      that PAP has better diagnostic efficiency as compared to MGG because the quality 
      index of PAP was 0.87 and that of MGG was 0.74, the quality index of AO was 0.9, 
      and that of DAPI was found to be 0.82, hence proving AO to be better in 
      comparison with DAPI. CONCLUSION: We found that among all the four stains used AO 
      is reliable, has greater efficacy, and can be easily performed with less cost. 
      Despite the difficulties mentioned in this study, the progress in the field of 
      fluorescence microscopy is expected to be rapid in the following years.
CI  - (c) 2021 Cytopathology Foundation Inc, Published by Scientific Scholar.
FAU - Warke, Darshana Jayant
AU  - Warke DJ
AD  - Department of Oral and Maxillofacial Pathology, Vedantaa Institute of Medical 
      Sciences, Mumbai, Maharashtra, India.
FAU - Khot, Komal Paresh
AU  - Khot KP
AD  - Department of Oral Pathology, YMT Dental College, Mumbai, Maharashtra, India.
LA  - eng
PT  - Journal Article
DEP - 20210618
PL  - United States
TA  - Cytojournal
JT  - CytoJournal
JID - 101231642
PMC - PMC8247698
OTO - NOTNLM
OT  - 4'
OT  - 6-diamidino-2-phenyl indole
OT  - Acridine orange
OT  - Fluorescence microscopy
OT  - Premalignant conditions
OT  - Premalignant lesions
COIS- No competing interests.
EDAT- 2021/07/06 06:00
MHDA- 2021/07/06 06:01
PMCR- 2021/06/18
CRDT- 2021/07/05 10:02
PHST- 2020/06/11 00:00 [received]
PHST- 2021/05/28 00:00 [accepted]
PHST- 2021/07/05 10:02 [entrez]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/07/06 06:01 [medline]
PHST- 2021/06/18 00:00 [pmc-release]
AID - 10.25259/Cytojournal_39_2020 [pii]
AID - 10.25259/Cytojournal_39_2020 [doi]
PST - epublish
SO  - Cytojournal. 2021 Jun 18;18:14. doi: 10.25259/Cytojournal_39_2020. eCollection 
      2021.