PMID- 34222184
OWN - NLM
STAT- MEDLINE
DCOM- 20210804
LR  - 20220531
IS  - 2296-2565 (Electronic)
IS  - 2296-2565 (Linking)
VI  - 9
DP  - 2021
TI  - Antimicrobial Resistance Profiles of Coagulase-Negative Staphylococci in 
      Community-Based Healthy Individuals in Germany.
PG  - 684456
LID - 10.3389/fpubh.2021.684456 [doi]
LID - 684456
AB  - Coagulase-negative staphylococci (CoNS) are common opportunistic pathogens, but 
      also ubiquitous human and animal commensals. Infection-associated CoNS from 
      healthcare environments are typically characterized by pronounced antimicrobial 
      resistance (AMR) including both methicillin- and multidrug-resistant isolates. 
      Less is known about AMR patterns of CoNS colonizing the general population. Here 
      we report on AMR in commensal CoNS recovered from 117 non-hospitalized volunteers 
      in a region of Germany with a high livestock density. Among the 69 individuals 
      colonized with CoNS, 29 had reported contacts to either companion or farm 
      animals. CoNS were selectively cultivated from nasal swabs, followed by species 
      definition by 16S rDNA sequencing and routine antibiotic susceptibility testing. 
      Isolates displaying phenotypic AMR were further tested by PCR for presence of 
      selected AMR genes. A total of 127 CoNS were isolated and Staphylococcus 
      epidermidis (75%) was the most common CoNS species identified. Nine isolates (7%) 
      were methicillin-resistant (MR) and carried the mecA gene, with seven individuals 
      (10%) being colonized with at least one MR-CoNS isolate. While resistance against 
      gentamicin, phenicols and spectinomycin was rare, high resistance rates were 
      found against tetracycline (39%), erythromycin (33%) and fusidic acid (24%). In 
      the majority of isolates, phenotypic resistance could be associated with 
      corresponding AMR gene detection. Multidrug-resistance (MDR) was observed in 23% 
      (29/127) of the isolates, with 33% (23/69) of the individuals being colonized 
      with MDR-CoNS. The combined data suggest that MR- and MDR-CoNS are present in the 
      community, with previous animal contact not significantly influencing the risk of 
      becoming colonized with such isolates.
CI  - Copyright (c) 2021 Marincola, Liong, Schoen, Abouelfetouh, Hamdy, Wencker, 
      Marciniak, Becker, Kock and Ziebuhr.
FAU - Marincola, Gabriella
AU  - Marincola G
AD  - Institute of Molecular Infection Biology, University of Wurzburg, Wurzburg, 
      Germany.
FAU - Liong, Olivia
AU  - Liong O
AD  - Institute of Molecular Infection Biology, University of Wurzburg, Wurzburg, 
      Germany.
FAU - Schoen, Christoph
AU  - Schoen C
AD  - Institute of Hygiene and Microbiology, University of Wurzburg, Wurzburg, Germany.
FAU - Abouelfetouh, Alaa
AU  - Abouelfetouh A
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria 
      University, Alexandria, Egypt.
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, AlAlamein 
      International University, AlAlamein, Egypt.
FAU - Hamdy, Aisha
AU  - Hamdy A
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria 
      University, Alexandria, Egypt.
FAU - Wencker, Freya D R
AU  - Wencker FDR
AD  - Institute of Molecular Infection Biology, University of Wurzburg, Wurzburg, 
      Germany.
FAU - Marciniak, Tessa
AU  - Marciniak T
AD  - Institute of Molecular Infection Biology, University of Wurzburg, Wurzburg, 
      Germany.
AD  - Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for 
      Infection Research (HZI), Wurzburg, Germany.
FAU - Becker, Karsten
AU  - Becker K
AD  - Friedrich Loeffler-Institute of Medical Microbiology, University Medicine 
      Greifswald, Greifswald, Germany.
FAU - Kock, Robin
AU  - Kock R
AD  - Deutsches Rotes Kreuz (DRK) Kliniken Berlin, Berlin, Germany.
AD  - Institute of Hygiene, University Hospital Munster, Munster, Germany.
FAU - Ziebuhr, Wilma
AU  - Ziebuhr W
AD  - Institute of Molecular Infection Biology, University of Wurzburg, Wurzburg, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210617
PL  - Switzerland
TA  - Front Public Health
JT  - Frontiers in public health
JID - 101616579
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coagulase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Coagulase/genetics
MH  - *Drug Resistance, Bacterial/genetics
MH  - Germany/epidemiology
MH  - Humans
MH  - *Staphylococcal Infections/drug therapy
PMC - PMC8247762
OTO - NOTNLM
OT  - Germany
OT  - One Health
OT  - antimicrobial resistance
OT  - coagulase-negative staphylococci
OT  - community settings
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/06 06:00
MHDA- 2021/08/05 06:00
PMCR- 2021/06/17
CRDT- 2021/07/05 10:14
PHST- 2021/03/23 00:00 [received]
PHST- 2021/05/24 00:00 [accepted]
PHST- 2021/07/05 10:14 [entrez]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/08/05 06:00 [medline]
PHST- 2021/06/17 00:00 [pmc-release]
AID - 10.3389/fpubh.2021.684456 [doi]
PST - epublish
SO  - Front Public Health. 2021 Jun 17;9:684456. doi: 10.3389/fpubh.2021.684456. 
      eCollection 2021.