PMID- 34224092
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210721
IS  - 2194-0509 (Print)
IS  - 2194-0517 (Electronic)
IS  - 2194-0517 (Linking)
VI  - 10
IP  - 2
DP  - 2021 Jun
TI  - Cell encapsulated and microenvironment modulating microbeads containing alginate 
      hydrogel system for bone tissue engineering.
PG  - 131-150
LID - 10.1007/s40204-021-00158-3 [doi]
AB  - Functional tissue regeneration using synthetic biomaterials requires 
      proliferation and heterotypic differentiation of stem/progenitor cells within a 
      specialized heterogeneous (biophysical-biochemical) microenvironment. The current 
      techniques have limitations to develop synthetic hydrogels, mimicking native 
      extracellular matrix porosity along with heterogeneous microenvironmental cues of 
      matrix mechanics, degradability, microstructure and cell-cell interactions. Here, 
      we have developed a microenvironment modulating system to fabricate in situ 
      porous hydrogel matrix with two or more distinct tailored microenvironmental 
      niches within microbeads and the hydrogel matrix for multicellular tissue 
      regeneration. Electrosprayed pectin-gelatin blended microbeads and crosslinked 
      alginate hydrogel system help to tailor microenvironmental niches of encapsulated 
      cells where two different cells are surrounded by a specific microenvironment. 
      The effect of different microenvironmental parameters associated with the 
      microbead/hydrogel matrix was evaluated using human umbilical-cord mesenchymal 
      stem cells (hUCMSCs). The osteogenic differentiation of hUCMSCs in the hydrogel 
      matrix was evaluated for bone tissue regeneration. This will be the first report 
      on microenvironment modulating microbead-hydrogel system to encapsulate two/more 
      types of cells in a hydrogel, where each cell is surrounded with distinct niches 
      for heterogeneous tissue regeneration.
CI  - (c) 2021. Islamic Azad University.
FAU - Veernala, Induvahi
AU  - Veernala I
AD  - Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, 
      Kandi, Telangana, India.
FAU - Roopmani, Purandhi
AU  - Roopmani P
AD  - Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, 
      Kandi, Telangana, India.
FAU - Singh, Ruby
AU  - Singh R
AD  - Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, 
      Kandi, Telangana, India.
FAU - Hasan, Uzma
AU  - Hasan U
AD  - Department of Biotechnology, Indian Institute of Technology, Hyderabad, Kandi, 
      Telangana, India.
FAU - Giri, Jyotsnendu
AU  - Giri J
AD  - Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, 
      Kandi, Telangana, India. enarm@bme.iith.ac.in.
LA  - eng
GR  - BT/RLF/Re-entry/21/2013/Department of Biotechnology , Ministry of Science and 
      Technology/
GR  - BT/PR23662/NNT/28/1300/2017/Department of Biotechnology , Ministry of Science and 
      Technology/
GR  - BT/PR24271/TDS/121/40/2017/Department of Biotechnology , Ministry of Science and 
      Technology/
GR  - SR/NM/-NS-1364/2014(G)/Department of Science and Technology, Ministry of Science 
      and Technology/
GR  - SERB-ECR/2015/00040, SB/S3/CE/048/2015/Science and Engineering Research Board/
PT  - Journal Article
DEP - 20210705
PL  - Germany
TA  - Prog Biomater
JT  - Progress in biomaterials
JID - 101656983
PMC - PMC8271062
OTO - NOTNLM
OT  - Electrospraying
OT  - Microenvironment modulating hydrogel
OT  - Microenvironment modulating microbead
OT  - Stem cells
OT  - Tissue engineering
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/06 06:00
MHDA- 2021/07/06 06:01
PMCR- 2021/07/05
CRDT- 2021/07/05 12:25
PHST- 2020/12/09 00:00 [received]
PHST- 2021/05/22 00:00 [accepted]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/07/06 06:01 [medline]
PHST- 2021/07/05 12:25 [entrez]
PHST- 2021/07/05 00:00 [pmc-release]
AID - 10.1007/s40204-021-00158-3 [pii]
AID - 158 [pii]
AID - 10.1007/s40204-021-00158-3 [doi]
PST - ppublish
SO  - Prog Biomater. 2021 Jun;10(2):131-150. doi: 10.1007/s40204-021-00158-3. Epub 2021 
      Jul 5.