PMID- 34224230
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 49
IP  - 4
DP  - 2021
TI  - Cross-reactivity of Can f 1 with Syrian hamster and Fel d 1 in children.
PG  - 155-161
LID - 10.15586/aei.v49i4.206 [doi]
AB  - Introduction and objectives: With increasing pet allergies among pediatric 
      patients, the need for precise environmental care is increasing. We investigated 
      the clinical, immunological, and environmental characteristics of pediatric 
      patients sensitized to a dog to evaluate the cross-antigenicity of canine 
      lipocalin Can f 1 with feline lipocalin Fel d 1 and Syrian hamster 
      extract.Materials and methods: The protein fractions of the processed and 
      commercial Syrian hamster extracts were compared using sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An enzyme-linked 
      immunosorbent assay (ELISA) inhibition test was performed on Can f 1, Fel d 1, 
      and processed Syrian hamster extract, and the antigen-specific immunoglobulin E 
      (IgE)-binding capacity for each antigen was analyzed using serum samples from 
      patients.Results: Twelve of 19 patients with a median age of 40.5 months were 
      symptomatic when exposed to dogs. Eleven (91.7%) patients showed a positive IgE 
      response to Can f 1. Two patients were positive for Fel d 1-specific IgE 
      antibody, and one was positive for hamster-specific IgE antibody. SDS-PAGE 
      confirmed the presence of different patterns of protein bands between the 
      commercial and processed hamster extracts. There was no cross-antigenicity among 
      Can f 1, Fel d 1, and processed Syrian hamster extract.
FAU - Jeongmin, Lee
AU  - Jeongmin L
AD  - Department of Pediatrics, Yonsei University, Wonju College of Medicine, Wonju, 
      Korea.
AD  - Department of Pediatrics, Ajou University Graduate School of Medicine, Suwon, 
      Korea.
FAU - Se-Ah, Jeon
AU  - Se-Ah J
AD  - Department of Pediatrics, Ajou University Graduate School of Medicine, Suwon, 
      Korea.
FAU - Sooyoung, Lee
AU  - Sooyoung L
AD  - Department of Pediatrics, Ajou University Graduate School of Medicine, Suwon, 
      Korea; jsjs87@ajou.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20210701
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (Allergens)
RN  - 0 (Lipocalins)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Cats
MH  - Child, Preschool
MH  - Cricetinae
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin E
MH  - Lipocalins
MH  - Mesocricetus
MH  - Plant Extracts
OTO - NOTNLM
OT  - Can f 1
OT  - Syrian hamster
OT  - children
OT  - cross-reactivity
OT  - dog allergy
COIS- This study was supported by the 2012 MSD research grant of the Korean Academy of 
      Pediatric Allergy and Respiratory Disease.
EDAT- 2021/07/06 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/07/05 12:34
PHST- 2021/01/29 00:00 [received]
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/07/05 12:34 [entrez]
PHST- 2021/07/06 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
AID - 10.15586/aei.v49i4.206 [doi]
PST - epublish
SO  - Allergol Immunopathol (Madr). 2021 Jul 1;49(4):155-161. doi: 
      10.15586/aei.v49i4.206. eCollection 2021.