PMID- 34225677
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210709
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 22
IP  - 1
DP  - 2021 Jul 6
TI  - Using carrot centromeric repeats to study karyotype relationships in the genus 
      Daucus (Apiaceae).
PG  - 508
LID - 10.1186/s12864-021-07853-2 [doi]
LID - 508
AB  - BACKGROUND: In the course of evolution, chromosomes undergo evolutionary changes; 
      thus, karyotypes may differ considerably among groups of organisms, even within 
      closely related taxa. The genus Daucus seems to be a promising model for 
      exploring the dynamics of karyotype evolution. It comprises some 40 wild species 
      and the cultivated carrot, a crop of great economic significance. However, Daucus 
      species are very diverse morphologically and genetically, and despite extensive 
      research, the taxonomic and phylogenetic relationships between them have still 
      not been fully resolved. Although several molecular cytogenetic studies have been 
      conducted to investigate the chromosomal structure and karyotype evolution of 
      carrot and other Daucus species, detailed karyomorphological research has been 
      limited to carrot and only a few wild species. Therefore, to better understand 
      the karyotype relationships within Daucus, we (1) explored the chromosomal 
      distribution of carrot centromeric repeats (CentDc) in 34 accessions of Daucus 
      and related species by means of fluorescence in situ hybridization (FISH) and (2) 
      performed detailed karyomorphological analysis in 16 of them. RESULTS: We 
      determined the genomic organization of CentDc in 26 accessions of Daucus 
      (belonging to both Daucus I and II subclades) and one accession of closely 
      related species. The CentDc repeats were present in the centromeric regions of 
      all chromosomes of 20 accessions (representing 11 taxa). In the other Daucus 
      taxa, the number of chromosome pairs with CentDc signals varied depending on the 
      species, yet their centromeric localization was conserved. In addition, precise 
      chromosome measurements performed in 16 accessions showed the inter- and 
      intraspecific karyological relationships among them. CONCLUSIONS: The presence of 
      the CentDc repeats in the genomes of taxa belonging to both Daucus subclades and 
      one outgroup species indicated the ancestral status of the repeat. The results of 
      our study provide useful information for further evolutionary, cytotaxonomic, and 
      phylogenetic research on the genus Daucus and may contribute to a better 
      understanding of the dynamic evolution of centromeric satellites in plants.
FAU - Kadluczka, Dariusz
AU  - Kadluczka D
AUID- ORCID: 0000-0002-6890-9785
AD  - Department of Plant Biology and Biotechnology, Faculty of Biotechnology and 
      Horticulture, University of Agriculture in Krakow, al. 29 Listopada 54, 31-425, 
      Krakow, Poland. darek.kadluczka@gmail.com.
FAU - Grzebelus, Ewa
AU  - Grzebelus E
AUID- ORCID: 0000-0003-2067-585X
AD  - Department of Plant Biology and Biotechnology, Faculty of Biotechnology and 
      Horticulture, University of Agriculture in Krakow, al. 29 Listopada 54, 31-425, 
      Krakow, Poland. ewa.grzebelus@urk.edu.pl.
LA  - eng
PT  - Journal Article
DEP - 20210706
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - *Apiaceae
MH  - *Daucus carota/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Phylogeny
PMC - PMC8259371
OTO - NOTNLM
OT  - Crop wild relatives
OT  - Cytotaxonomy
OT  - Karyomorphology
OT  - Molecular cytogenetics
OT  - Plant chromosomes
COIS- The authors declare that they have no competing interests.
EDAT- 2021/07/07 06:00
MHDA- 2021/07/08 06:00
PMCR- 2021/07/06
CRDT- 2021/07/06 05:35
PHST- 2021/04/29 00:00 [received]
PHST- 2021/06/24 00:00 [accepted]
PHST- 2021/07/06 05:35 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2021/07/06 00:00 [pmc-release]
AID - 10.1186/s12864-021-07853-2 [pii]
AID - 7853 [pii]
AID - 10.1186/s12864-021-07853-2 [doi]
PST - epublish
SO  - BMC Genomics. 2021 Jul 6;22(1):508. doi: 10.1186/s12864-021-07853-2.