PMID- 34225728
OWN - NLM
STAT- MEDLINE
DCOM- 20211230
LR  - 20231213
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 16
IP  - 1
DP  - 2021 Jul 5
TI  - CCND1 copy number increase and cyclin D1 expression in acral melanoma: a 
      comparative study of fluorescence in situ hybridization and immunohistochemistry 
      in a Chinese cohort.
PG  - 60
LID - 10.1186/s13000-021-01116-0 [doi]
LID - 60
AB  - BACKGROUND: CCND1 copy number increase is characteristic of acral melanoma and is 
      useful in distinguishing benign and malignant acral melanocytic lesions. Increase 
      of the gene copy number may result in protein overexpression. This raises the 
      possibility that detection of high expression of cyclin D1 by 
      immunohistochemistry (IHC) may be used as a surrogate for direct evaluation of 
      increase in the CCND1 gene copy number. METHODS: We examined increases in CCND1 
      copy number with fluorescence in situ hybridization (FISH), and examined cyclin 
      D1 protein expression with IHC in 61 acral melanomas. RESULTS: Using FISH, 29 
      acral melanomas (29/61, 47.5%) showed increase in the CCND1 copy number, 
      including 8 (8/61, 13.1%) which showed low-level increase in the CCND1 copy 
      number and 21 (21/61, 34.4%) with high-level increase in the CCND1 copy number. 
      By analysis of IHC, the median IHC score was 15% (range: 1-80%) in acral 
      melanomas with no CCND1 copy number alteration. In acral melanomas with low-level 
      CCND1 copy number increase, the median IHC score was 25% (range: 3-90%). In acral 
      melanomas with high-level CCND1 copy number increase, the median IHC score was 
      60% (range: 1-95%). Comparing FISH and IHC, cyclin D1 protein expression level 
      has no corelation with the CCND1 copy number in acral melanomas which have no 
      CCND1 copy number alteration and low-level CCND1 copy number increase 
      (P = 0.108). Cyclin D1 protein expression level correlated positively with CCND1 
      copy number in acral melanomas with high-level CCND1 copy number increase 
      (P = 0.038). The sensitivity, specificity and positive predictive value of using 
      cyclin D1 IHC to predict CCND1 FISH result was 72.4, 62.5 and 63.6%. Increase in 
      CCND1 copy number was associated with Breslow thickness in invasive acral 
      melanoma. CONCLUSION: High-level increase in the CCND1 copy number can induce 
      high cyclin D1 protein expression in acral melanomas. However low-level increase 
      and normal CCND1 copy number have no obvious correlation with protein expression. 
      Cyclin D1 IHC cannot serve as a surrogate for CCND1 FISH in acral melanomas.
FAU - Liu, Jianying
AU  - Liu J
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Yu, Wenjuan
AU  - Yu W
AD  - Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Qi, Shuangshuang
AU  - Qi S
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Du, Juan
AU  - Du J
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Ma, Xiaolong
AU  - Ma X
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Zheng, Jie
AU  - Zheng J
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
FAU - Su, Jing
AU  - Su J
AUID- ORCID: 0000-0002-4810-6096
AD  - Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking 
      University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China. 
      sujing@bjmu.edu.cn.
LA  - eng
GR  - 81802245/National Natural Science Foundation of China/
PT  - Comparative Study
PT  - Journal Article
DEP - 20210705
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - China
MH  - Cohort Studies
MH  - Cyclin D1/genetics/*metabolism
MH  - DNA Copy Number Variations/*genetics
MH  - Female
MH  - Gene Amplification/genetics
MH  - Gene Dosage
MH  - Humans
MH  - *Immunohistochemistry/methods
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Melanoma/*genetics
MH  - Middle Aged
MH  - Skin Neoplasms/*genetics
MH  - Melanoma, Cutaneous Malignant
PMC - PMC8259423
OTO - NOTNLM
OT  - Acral melanoma
OT  - CCND1 (cyclin D1)
OT  - Gene copy number increase
OT  - Protein expression
COIS- The authors declare that they have no competing interests.
EDAT- 2021/07/07 06:00
MHDA- 2021/12/31 06:00
PMCR- 2021/07/05
CRDT- 2021/07/06 05:38
PHST- 2021/02/14 00:00 [received]
PHST- 2021/06/07 00:00 [accepted]
PHST- 2021/07/06 05:38 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2021/12/31 06:00 [medline]
PHST- 2021/07/05 00:00 [pmc-release]
AID - 10.1186/s13000-021-01116-0 [pii]
AID - 1116 [pii]
AID - 10.1186/s13000-021-01116-0 [doi]
PST - epublish
SO  - Diagn Pathol. 2021 Jul 5;16(1):60. doi: 10.1186/s13000-021-01116-0.