PMID- 34227231
OWN - NLM
STAT- MEDLINE
DCOM- 20220317
LR  - 20220317
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 24
IP  - 2
DP  - 2022 Feb
TI  - Functional characterization of a high-affinity iron transporter (PiFTR) from the 
      endophytic fungus Piriformospora indica and its role in plant growth and 
      development.
PG  - 689-706
LID - 10.1111/1462-2920.15659 [doi]
AB  - Iron (Fe) is a micronutrient required for plant growth and development; however, 
      most Fe forms in soil are not readily available to plants, resulting in low 
      Fe contents in plants and, thereby, causing Fe deficiency in humans. 
      Biofortification through plant-fungal co-cultivation might be a sustainable 
      approach to increase crop Fe contents. Therefore, we aimed to examine the role of 
      a Piriformospora indica Fe transporter on rice Fe uptake under low Fe conditions. 
      A high-affinity Fe transporter (PiFTR) from P. indica was identified and 
      functionally characterized. PiFTR fulfilled all criteria expected of a functional 
      Fe transporter under Fe-limited conditions. Additionally, PiFTR expression was 
      induced when P. indica was grown under low Fe conditions, and PiFTR complemented 
      a yeast mutant lacking Fe transport. A knockdown (KD) P. indica strain was 
      created via RNA interference to understand the physiological role of PiFTR. We 
      observed that the KD-PiFTR-P. indica strain transported a significantly lower 
      amount of Fe to colonized rice (Oryza sativa) than the wild type (WT) P. indica. 
      WT P. indica-colonized rice plants were healthier and performed significantly 
      better than KD-PiFTR-P. indica-colonized rice plants. Our study offers potential 
      avenues for an agronomically sound amelioration of plant growth in low Fe 
      environments.
CI  - (c) 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Verma, Nidhi
AU  - Verma N
AUID- ORCID: 0000-0003-0643-8858
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Narayan, Om Prakash
AU  - Narayan OP
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Prasad, Durga
AU  - Prasad D
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Jogawat, Abhimanyu
AU  - Jogawat A
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Panwar, Sneh Lata
AU  - Panwar SL
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
FAU - Dua, Meenakshi
AU  - Dua M
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
FAU - Johri, Atul Kumar
AU  - Johri AK
AUID- ORCID: 0000-0001-9480-3063
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210722
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - E1UOL152H7 (Iron)
RN  - Serendipita indica
SB  - IM
MH  - *Basidiomycota/metabolism
MH  - Humans
MH  - Iron/metabolism
MH  - *Oryza/microbiology
MH  - Plant Development
MH  - Plant Roots/microbiology
EDAT- 2021/07/07 06:00
MHDA- 2022/03/18 06:00
CRDT- 2021/07/06 06:58
PHST- 2021/07/01 00:00 [revised]
PHST- 2020/05/10 00:00 [received]
PHST- 2021/07/02 00:00 [accepted]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2022/03/18 06:00 [medline]
PHST- 2021/07/06 06:58 [entrez]
AID - 10.1111/1462-2920.15659 [doi]
PST - ppublish
SO  - Environ Microbiol. 2022 Feb;24(2):689-706. doi: 10.1111/1462-2920.15659. Epub 
      2021 Jul 22.