PMID- 34227315
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20220915
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 39
IP  - 3
DP  - 2021 Mar
TI  - [Determination of amanita peptide toxins in human urine by TurboFlow online 
      clean-up-liquid chromatography-tandem mass spectrometry].
PG  - 338-345
LID - 10.3724/SP.J.1123.2020.06005 [doi]
AB  - Amanita peptide toxins are cyclic polypeptide mushroom toxins that can cause 
      acute liver damage. The fatality rate associated with these toxins is very high. 
      Monitoring the concentration of amanita peptide toxins in human urine can provide 
      valuable information for early clinical diagnosis and treatment. Therefore, a 
      TurboFlow online clean-up-liquid chromatography-triple quadrupole mass 
      spectrometry (TF-LC-MS/MS) method was established for the simultaneous 
      quantitative determination of five amanita peptide toxins (alpha-amanitin, 
      beta-amanitin, gamma-amanitin, phallacidin, and phalloidin) in human urine. After 
      pre-treatment with high-speed centrifugation, urine samples were analyzed using 
      TF-LC-MS/MS. The main factors influencing purification efficiency, including the 
      TF column, loading solution, eluting solution, transfer flow, and transfer time, 
      were optimized in this study. Under the optimized experimental conditions, the 
      analytes were purified using a TurboFlow(TM) Cyclone column (50 mmx0.5 mm) and 
      separated on a Hypersil GOLD C(18) column (100 mmx2.1 mm) using the mobile phases 
      of methanol and 4 mmol/L aqueous ammonium acetate solution with gradient elution. 
      The analytes were detected in selected reaction monitoring (SRM) mode via 
      positive electrospray ionization. Matrix-matched external standard calibration 
      was used for quantitation. The linear range of the method ranged from 1.0 mug/L to 
      50.0 mug/L for all five amanita peptide toxins, with correlation coefficients 
      (r(2)) higher than 0.997. The limits of detection were 0.15-0.3 mug/L and the 
      limits of quantification (LOQs) were 0.5-1.0 mug/L for the five amanita peptide 
      toxins in urine. The intra-day and inter-day recoveries of amanita peptide toxins 
      were 87.0%-108.6% and 86.8%-112.7%, respectively, at the spiked levels of 2.0, 
      5.0, and 10.0 mug/L. The intra-day and inter-day relative standard deviations 
      (RSDs) were less than 14.5%. The method is accurate, rapid, sensitive, easy to 
      operate, and can satisfy the requirements of public health emergency testing or 
      clinical poisoning testing.
FAU - Fang, Li
AU  - Fang L
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province (Zhoushan 
      Municipal Center for Disease Control and Prevention), Zhoushan 316021, China.
FAU - Qiu, Fengmei
AU  - Qiu F
AD  - Daishan Center for Disease Control and Prevention, Daishan 316200, China.
FAU - Yu, Xinwei
AU  - Yu X
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province (Zhoushan 
      Municipal Center for Disease Control and Prevention), Zhoushan 316021, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Amanita/*chemistry
MH  - *Chromatography, Liquid
MH  - Humans
MH  - Mushroom Poisoning/*diagnosis
MH  - *Mycotoxins/urine
MH  - *Tandem Mass Spectrometry
PMC - PMC9403809
OTO - NOTNLM
OT  - amanita peptide toxins
OT  - liquid chromatography-tandem mass spectrometry (LC-MS/MS)
OT  - mushroom toxins
OT  - online-cleanup
OT  - urine
EDAT- 2021/07/07 06:00
MHDA- 2021/07/23 06:00
PMCR- 2021/03/08
CRDT- 2021/07/06 07:00
PHST- 2021/07/06 07:00 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2021/03/08 00:00 [pmc-release]
AID - 1000-8713-39-3-338 [pii]
AID - 10.3724/SP.J.1123.2020.06005 [doi]
PST - ppublish
SO  - Se Pu. 2021 Mar;39(3):338-345. doi: 10.3724/SP.J.1123.2020.06005.