PMID- 34227338
OWN - NLM
STAT- MEDLINE
DCOM- 20210727
LR  - 20220915
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 39
IP  - 5
DP  - 2021 May
TI  - [Design and application of special solid phase extraction column for three 
      cannabinol compounds in hemp].
PG  - 534-540
LID - 10.3724/SP.J.1123.2020.09025 [doi]
AB  - Cannabidiol (CBD), cannabinol (CBN), and Delta9-tetrahydrocannabinol (THC) are the 
      most important components of hemp, whose concentrations determine the properties 
      and applications of hemp. Hemp contains a large number of impurities, which must 
      be removed from the extracting solution before determining the cannabinol 
      contents by ultra-high performance liquid chromatography (UHPLC). Neutral 
      alumina, magnesium silicate, and graphitized carbon black have different surface 
      characteristics when used as adsorbents. The removal rates of pigments, total 
      sugar, total fatty acid glyceride, and metal ions as well as the recoveries of 
      the three cannabinols in the extraction solution were evaluated. The amounts of 
      neutral alumina, magnesium silicate, and graphitized carbon black were 1.80 g, 
      0.15 g, and 0.05 g, respectively. The three adsorbents were mixed well and packed 
      into a polypropylene pipe to prepare a special 2 g/6 mL solid phase extraction 
      (SPE) column for determining the three cannabinol compounds in hemp. The chemical 
      components of the hemp flowers and leaves were extracted with an ethyl 
      acetate/methanol (9ratio1, v/v) mixture. After the extracting solution was allowed to 
      pass through the SPE column, the recoveries of CBD, CBN, and Delta9-THC were 98.9%, 
      95.7%, and 99.2%, respectively. The removal rates of xanthophyll, chlorophyll a, 
      and chlorophyll were 96.3%, 99.2%, and 95.5%, respectively. The removal rates of 
      total sugar, total fatty glyceride, and metal ions were 98.5%, 96.9%, and 85.4%, 
      respectively. In this study, the chromatographic conditions for analyzing the 
      three cannabinol compounds were optimized. The cannabinol compounds were 
      separated within 10 min on an Eclipse Plus C18 column (50 mmx2.1 mm, 1.8 mum) 
      using a mobile phase consisting of 1% (v/v) acetic acid and acetonitrile (30ratio70, 
      v/v) at a flow rate of 0.5 mL/min. The detection wavelength was set at 210 nm 
      with a diode array detector, and the sample injection volume was 1 muL. Good 
      linear relationships were observed between the mass peak areas and mass 
      concentrations of CBD, CBN, and Delta9-THC in the range of 0.5-50 mg/L. The 
      corresponding correlation coefficients (R(2)) were 0.9983, 0.9995, and 0.9981, 
      while the detection limits were 0.45 mug/L, 0.53 mug/L, and 0.38 mug/L. The 
      recoveries of CBD, CBN, and Delta9-THC were 90.3%-96.9%, 93.7%-95.6%, and 
      90.8%-96.1%, with relative standard deviations (RSDs) of 2.2%-6.1%, 4.1%-8.0%, 
      and 2.4%-4.8%, respectively. The results were satisfactory, demonstrating that 
      the special SPE column made of neutral alumina, magnesium silicate, and 
      graphitized carbon black was well suited for the determination of the three 
      cannabinol compounds in hemp.
FAU - Shen, Shuchang
AU  - Shen S
AD  - Center of Analytical and Testing, Qiqihar University, Qiqihar 161006, China.
FAU - Li, Shaohua
AU  - Li S
AD  - Center of Analytical and Testing, Qiqihar University, Qiqihar 161006, China.
FAU - Guo, Li
AU  - Guo L
AD  - Daqing Branches of Heilongjiang Academy of Agricutural Sciences, Daqing 163316, 
      China.
FAU - Lu, Weichao
AU  - Lu W
AD  - Center of Analytical and Testing, Qiqihar University, Qiqihar 161006, China.
FAU - Li, Qiushi
AU  - Li Q
AD  - Center of Analytical and Testing, Qiqihar University, Qiqihar 161006, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 19GBJ60SN5 (Cannabidiol)
RN  - 7J8897W37S (Dronabinol)
RN  - 7UYP6MC9GH (Cannabinol)
SB  - IM
MH  - *Cannabidiol/isolation & purification
MH  - Cannabinol/*isolation & purification
MH  - *Cannabis/chemistry
MH  - Dronabinol/*isolation & purification
MH  - Solid Phase Extraction
PMC - PMC9404009
OTO - NOTNLM
OT  - cannabidiol
OT  - cannabinol
OT  - hemp
OT  - special solid phase extraction column
OT  - ultra-high performance liquid chromatography (UHPLC)
OT  - Delta9-tetrahydrocannabinol;
EDAT- 2021/07/07 06:00
MHDA- 2021/07/28 06:00
PMCR- 2021/05/08
CRDT- 2021/07/06 07:00
PHST- 2021/07/06 07:00 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2021/07/28 06:00 [medline]
PHST- 2021/05/08 00:00 [pmc-release]
AID - 1000-8713-39-5-534 [pii]
AID - 10.3724/SP.J.1123.2020.09025 [doi]
PST - ppublish
SO  - Se Pu. 2021 May;39(5):534-540. doi: 10.3724/SP.J.1123.2020.09025.