PMID- 34227372
OWN - NLM
STAT- MEDLINE
DCOM- 20210729
LR  - 20220915
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 39
IP  - 7
DP  - 2021 Jul 8
TI  - [Simultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in 
      fermentation broth by high performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 744-749
LID - 10.3724/SP.J.1123.2020.09018 [doi]
AB  - An analytical method was established for the simultaneously determination the 
      pentostatin and 2'-amino-2'-deoxyadenosine contents in fermentation broth by high 
      performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After 
      high-speed centrifugation, aqueous solution dilution, vortex shock, and 
      microfiltration, the fermentation broth samples were analyzed by HPLC-MS/MS. The 
      samples were separated on a Waters Atlantis((R)) T3 column (100 mmx2.1 mm, 5 mum) 
      using a gradient elution program with 10 mmol/L ammonium formate (containing 0.1% 
      formic acid) and methanol (containing 0.02% formic acid) as the mobile phases. 
      Moreover, a chromatographic protection column (5 mmx2.1 mm, 5 mum) was added to 
      preserve the column efficiency. The flow rate, column temperature, and injection 
      volume were set at 0.3 mL/min, 25 ℃, and 10 muL, respectively. Qualitative and 
      quantitative analyses of the target compounds were performed using an ESI(+) 
      source. MS parameters such as the collision energies and tube lens offsets of 
      pentostatin and 2'-amino-2'-deoxyadenosine were optimized. The quantitative ion 
      pairs of pentostatin and 2'-amino-2'-deoxyadenosine were m/z 269.17>153.20 and 
      m/z 267.00>136.10, respectively; the corresponding collision energies were 11 V 
      and 18 V. The external standard method was used for quantitative analysis. The 
      established method was verified rigorously in terms of the linear range, limit of 
      detection, limit of quantification, recovery rate, and precision. Pentostatin and 
      2'-amino-2'-deoxyadenosine showed good linear relationships in the range of 
      1.0-250 mug/L. The correlation coefficients ranged from 0.9969 to 0.9996, and the 
      relative standard deviations (RSDs) ranged from 6.51% to 8.35% (n=8). This result 
      indicated good accuracy and exactitude in the detection of the pentostatin and 
      2'-amino-2'-deoxyadenosine. The recoveries (n=6) at three spiked levels (1.0, 
      5.0, and 25 mug/L) were in the ranges of 97.94%-104.46% and 89.96%-107.21% for the 
      pentostatin and 2'-amino-2'-deoxyadenosine, respectively; the corresponding RSDs 
      were in the ranges of 3.74%-4.88% and 4.81%-13.29%. The limits of detection 
      (LODs, S/N>/=3) and limits of quantification (LOQs, S/N>/=10) of the 
      2'-amino-2'-deoxyadenosine and pentostatin in the fermentation broth were 
      0.003-0.060 mug/L and 0.010-0.200 mug/L, respectively. The validated experimental 
      method was used for the detection of actual samples, viz. the stored multiple 
      pentostatin-producing mutagenic strains in our laboratory. The HPLC-MS/MS method 
      for the determination of the pentostatin and 2'-amino-2'-deoxyadenosine in 
      fermentation broth offered the advantages of small sampling volume, strong 
      maneuverability, good stability, and high sensitivity. Compared with previously 
      published methods, this systematically established and optimized method 
      significantly reduced the detection time, and matrix effects were well 
      suppressed. Moreover, the peak shape and stability of the target compounds were 
      greatly improved. This method provides a methodological basis and meaningful 
      reference for the detection of the pentostatin and 2'-amino-2'-deoxyadenosine in 
      fermentation broth.
FAU - Zhao, Minmin
AU  - Zhao M
AD  - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food 
      Science, Tianjin University of Commerce, Tianjin 300134, China.
FAU - Zhang, Hongyu
AU  - Zhang H
AD  - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food 
      Science, Tianjin University of Commerce, Tianjin 300134, China.
FAU - Lou, Tingting
AU  - Lou T
AD  - Animal, Plant and Foodstuffs Inspection Center of Tianjin Customs, Tianjin 
      300461, China.
FAU - Zhao, Kongxiang
AU  - Zhao K
AD  - Animal, Plant and Foodstuffs Inspection Center of Tianjin Customs, Tianjin 
      300461, China.
FAU - Wang, Suying
AU  - Wang S
AD  - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food 
      Science, Tianjin University of Commerce, Tianjin 300134, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Deoxyadenosines)
RN  - 10414-81-0 (2'-amino-2'-deoxyadenosine)
RN  - 395575MZO7 (Pentostatin)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Deoxyadenosines/*analysis
MH  - *Fermentation
MH  - Pentostatin/*analysis
MH  - Tandem Mass Spectrometry
PMC - PMC9404223
OTO - NOTNLM
OT  - 2'-amino-2'-deoxyadenosine
OT  - fermentation broth
OT  - high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
OT  - pentostatin
EDAT- 2021/07/07 06:00
MHDA- 2021/07/30 06:00
PMCR- 2021/07/08
CRDT- 2021/07/06 07:00
PHST- 2021/07/06 07:00 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2021/07/30 06:00 [medline]
PHST- 2021/07/08 00:00 [pmc-release]
AID - 1000-8713-39-7-744 [pii]
AID - 10.3724/SP.J.1123.2020.09018 [doi]
PST - ppublish
SO  - Se Pu. 2021 Jul 8;39(7):744-749. doi: 10.3724/SP.J.1123.2020.09018.