PMID- 34227932
OWN - NLM
STAT- MEDLINE
DCOM- 20220118
LR  - 20220118
IS  - 2057-5858 (Electronic)
IS  - 2057-5858 (Linking)
VI  - 7
IP  - 7
DP  - 2021 Jul
TI  - Comparative genomic analysis of Escherichia coli isolates from cases of bovine 
      clinical mastitis identifies nine specific pathotype marker genes.
LID - 10.1099/mgen.0.000597 [doi]
LID - 000597
AB  - Escherichia coli is a major causative agent of environmental bovine mastitis and 
      this disease causes significant economic losses for the dairy industry. There is 
      still debate in the literature as to whether mammary pathogenic E. coli (MPEC) is 
      indeed a unique E. coli pathotype, or whether this infection is merely an 
      opportunistic infection caused by any E. coli isolate being displaced from the 
      bovine gastrointestinal tract to the environment and, then, into the udder. In 
      this study, we conducted a thorough genomic analysis of 113 novel MPEC isolates 
      from clinical mastitis cases and 100 bovine commensal E. coli isolates. A 
      phylogenomic analysis indicated that MPEC and commensal E. coli isolates formed 
      clades based on common sequence types and O antigens, but did not cluster based 
      on mammary pathogenicity. A comparative genomic analysis of MPEC and commensal 
      isolates led to the identification of nine genes that were part of either the 
      core or the soft-core MPEC genome, but were not found in any bovine commensal 
      isolates. These apparent MPEC marker genes were genes involved with nutrient 
      intake and metabolism [adeQ, adenine permease; nifJ, pyruvate-flavodoxin 
      oxidoreductase; and yhjX, putative major facilitator superfamily (MFS)-type 
      transporter], included fitness and virulence factors commonly seen in 
      uropathogenic E. coli (pqqL, zinc metallopeptidase, and fdeC, intimin-like 
      adhesin, respectively), and putative proteins [yfiE, uncharacterized 
      helix-turn-helix-type transcriptional activator; ygjI, putative inner membrane 
      transporter; and ygjJ, putative periplasmic protein]. Further characterization of 
      these highly conserved MPEC genes may be critical to understanding the 
      pathobiology of MPEC.
FAU - Jung, Dongyun
AU  - Jung D
AD  - Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill 
      University, Sainte Anne de Bellevue, Quebec, Canada.
AD  - Mastitis Network, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
FAU - Park, Soyoun
AU  - Park S
AD  - Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill 
      University, Sainte Anne de Bellevue, Quebec, Canada.
AD  - Mastitis Network, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
FAU - Ruffini, Janina
AU  - Ruffini J
AD  - Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill 
      University, Sainte Anne de Bellevue, Quebec, Canada.
FAU - Dussault, Forest
AU  - Dussault F
AD  - Health Canada, Ottawa, Ontario, Canada.
FAU - Dufour, Simon
AU  - Dufour S
AD  - Mastitis Network, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
AD  - Regroupement FRQNT Op+Lait, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
AD  - Faculte de Medecine Veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, 
      Canada.
FAU - Ronholm, Jennifer
AU  - Ronholm J
AD  - Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill 
      University, Sainte Anne de Bellevue, Quebec, Canada.
AD  - Mastitis Network, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
AD  - Regroupement FRQNT Op+Lait, Saint-Hyacinthe, Quebec J2S 2M2, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microb Genom
JT  - Microbial genomics
JID - 101671820
RN  - 0 (O Antigens)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Escherichia coli/classification/*genetics/isolation & purification/*pathogenicity
MH  - Escherichia coli Infections/*pathology
MH  - Female
MH  - Genome, Bacterial/*genetics
MH  - Genomics/methods
MH  - Humans
MH  - Mammary Glands, Animal/microbiology/pathology
MH  - Mastitis, Bovine/*microbiology/pathology
MH  - O Antigens/genetics
MH  - Whole Genome Sequencing
PMC - PMC8477405
OTO - NOTNLM
OT  - comparative genomics
OT  - environmental bovine mastitis
OT  - mammary pathogenic Escherichia coli
OT  - whole-genome sequencing
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2021/07/07 06:00
MHDA- 2022/01/19 06:00
PMCR- 2021/07/06
CRDT- 2021/07/06 12:24
PHST- 2021/07/06 12:24 [entrez]
PHST- 2021/07/07 06:00 [pubmed]
PHST- 2022/01/19 06:00 [medline]
PHST- 2021/07/06 00:00 [pmc-release]
AID - 000597 [pii]
AID - 10.1099/mgen.0.000597 [doi]
PST - ppublish
SO  - Microb Genom. 2021 Jul;7(7):000597. doi: 10.1099/mgen.0.000597.