PMID- 34233202
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20240116
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 196
DP  - 2021 Sep 15
TI  - Binge ethanol drinking associated with sex-dependent plasticity of neurons in the 
      insula that project to the bed nucleus of the stria terminalis.
PG  - 108695
LID - S0028-3908(21)00250-1 [pii]
LID - 10.1016/j.neuropharm.2021.108695 [doi]
AB  - Modifications in brain regions that govern reward-seeking are thought to 
      contribute to persistent behaviors that are heavily associated with alcohol-use 
      disorder (AUD) including binge ethanol drinking. The bed nucleus of the stria 
      terminalis (BNST) is a critical node linked to both alcohol consumption and the 
      onset, maintenance and progression of adaptive anxiety and stress-related 
      disorders. Differences in anatomy, connectivity and receptor subpopulations, make 
      the BNST a sexually dimorphic region. Previous work indicates that the ventral 
      BNST (vBNST) receives input from the insular cortex (IC), a brain region involved 
      in processing the body's internal state. This IC-vBNST projection has also been 
      implicated in emotional and reward-seeking processes. Therefore, we examined the 
      functional properties of vBNST-projecting, IC neurons in male and female mice 
      that have undergone short-term ethanol exposure and abstinence using a voluntary 
      Drinking in the Dark paradigm (DID) paired with whole-cell slice 
      electrophysiology. First we show that IC neurons projected predominantly to the 
      vBNST. Next, our data show that short-term ethanol exposure and abstinence 
      enhanced excitatory synaptic strength onto vBNST-projecting, IC neurons in both 
      sexes. However, we observed diametrically opposing modifications in excitability 
      across sexes. In particular, short-term ethanol exposure resulted in increased 
      intrinsic excitability of vBNST-projecting, IC neurons in females but not in 
      males. Furthermore, in females, abstinence decreased the excitability of these 
      same neurons. Taken together these findings show that short-term ethanol 
      exposure, as well as the abstinence cause sex-related adaptations in 
      BNST-projecting, IC neurons.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Marino, Rosa A M
AU  - Marino RAM
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA.
FAU - Girven, Kasey S
AU  - Girven KS
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA; Program in Neuroscience, University of 
      Maryland Baltimore, Baltimore, MD, 21201, USA.
FAU - Figueiredo, Antonio
AU  - Figueiredo A
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA; Program in Neuroscience, University of 
      Maryland Baltimore, Baltimore, MD, 21201, USA.
FAU - Navarrete, Jovana
AU  - Navarrete J
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA.
FAU - Doty, Carolyn
AU  - Doty C
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA; Program in Neuroscience, University of 
      Maryland Baltimore, Baltimore, MD, 21201, USA.
FAU - Sparta, Dennis R
AU  - Sparta DR
AD  - Department of Anatomy and Neurobiology, University of Maryland School of 
      Medicine, Baltimore, MD, 21201, USA; Program in Neuroscience, University of 
      Maryland Baltimore, Baltimore, MD, 21201, USA. Electronic address: 
      dsparta@som.umaryland.edu.
LA  - eng
GR  - F31 DA047050/DA/NIDA NIH HHS/United States
GR  - R00 AA021417/AA/NIAAA NIH HHS/United States
GR  - R01 AA027516/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210704
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Central Nervous System Depressants)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcohol Abstinence
MH  - Animals
MH  - Binge Drinking/*metabolism/physiopathology
MH  - Central Nervous System Depressants/administration & dosage/pharmacology
MH  - Ethanol/administration & dosage/pharmacology
MH  - Female
MH  - Insular Cortex/*metabolism/physiopathology
MH  - Male
MH  - Mice
MH  - Neural Pathways
MH  - Neuronal Plasticity/*physiology
MH  - Neurons/*metabolism/physiology
MH  - Patch-Clamp Techniques
MH  - Septal Nuclei/*metabolism/physiopathology
MH  - Sex Characteristics
MH  - Sex Factors
PMC - PMC8928450
MID - NIHMS1723355
OTO - NOTNLM
OT  - BNST
OT  - Binge ethanol drinking
OT  - Electrophysiology
OT  - Insula
OT  - Sex difference
EDAT- 2021/07/08 06:00
MHDA- 2022/02/03 06:00
PMCR- 2022/09/15
CRDT- 2021/07/07 20:08
PHST- 2020/12/22 00:00 [received]
PHST- 2021/05/26 00:00 [revised]
PHST- 2021/06/30 00:00 [accepted]
PHST- 2021/07/08 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/07/07 20:08 [entrez]
PHST- 2022/09/15 00:00 [pmc-release]
AID - S0028-3908(21)00250-1 [pii]
AID - 10.1016/j.neuropharm.2021.108695 [doi]
PST - ppublish
SO  - Neuropharmacology. 2021 Sep 15;196:108695. doi: 10.1016/j.neuropharm.2021.108695. 
      Epub 2021 Jul 4.