PMID- 34233227
OWN - NLM
STAT- MEDLINE
DCOM- 20210831
LR  - 20210831
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 311
IP  - 6
DP  - 2021 Aug
TI  - Systemic bacterial infections affect dendritic cell development and function.
PG  - 151517
LID - S1438-4221(21)00046-1 [pii]
LID - 10.1016/j.ijmm.2021.151517 [doi]
AB  - Dendritic cells (DCs) are critical in host defense against infection. DC 
      depletion is an early event in the course of sepsis that may impair the host 
      defense mechanisms. Here, we addressed whether DC depletion and dysfunction are 
      pathogen-independent, mediated via pattern recognition receptors, and are due to 
      impaired DC development upon systemic infection with the Gram-negative bacterium 
      Escherichia coli and the Gram-positive pathogen Staphylococcus aureus. Infection 
      with E. coli and S. aureus led to reduced numbers of splenic DC subsets and of DC 
      progenitors in the bone marrow (BM) with this effect persisting significantly 
      longer in mice infected with S. aureus than with E. coli. The reduction of DC 
      subsets and their progenitors was mainly TLR-independent as was the 
      infection-induced monopoiesis. Moreover, de novo DC development was impaired in 
      mice infected with S. aureus, and BM cells from E. coli or S. aureus infected 
      mice favored macrophage differentiation in vitro. As a consequence of reduced DC 
      numbers and their reduced expression of MHC II less CD4(+) and CD8(+) T cells, 
      especially Th1 and IFN-gamma producing CD8(+) T cells, could be detected in S. aureus 
      compared to E. coli infected mice. These differences are reflected in the rapid 
      killing of E. coli as opposed to an increase in bacterial load in S. aureus. In 
      summary, our study supports the idea that systemic bacterial infections generally 
      affect the number and development of DCs and thereby the T cell responses, but 
      the magnitude is pathogen-dependent.
CI  - Copyright (c) 2021 The Author(s). Published by Elsevier GmbH.. All rights reserved.
FAU - Bieber, Kristin
AU  - Bieber K
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany.
FAU - Gunter, Manina
AU  - Gunter M
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany; 
      Research Group "Dendritic Cells in Infection and Cancer", German Cancer Research 
      Center (DKFZ), Heidelberg, Germany.
FAU - Pasquevich, Karina A
AU  - Pasquevich KA
AD  - Instituto de Investigaciones Biotecnologicas-(IIBio), Universidad Nacional de San 
      Martin-CONICET, Argentina.
FAU - Autenrieth, Stella E
AU  - Autenrieth SE
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany; 
      Research Group "Dendritic Cells in Infection and Cancer", German Cancer Research 
      Center (DKFZ), Heidelberg, Germany. Electronic address: 
      Stella.Autenrieth@medizin.uni-tuebingen.de.
LA  - eng
PT  - Journal Article
DEP - 20210630
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes
MH  - Cell Differentiation
MH  - Dendritic Cells
MH  - Escherichia coli
MH  - Mice
MH  - *Sepsis
MH  - *Staphylococcal Infections
MH  - Staphylococcus aureus
OTO - NOTNLM
OT  - Dendritic cells
OT  - Development
OT  - Escherichia coli
OT  - Infection
OT  - Innate immune defense
OT  - Monocytes
OT  - Staphylococcus aureus
OT  - TLR
EDAT- 2021/07/08 06:00
MHDA- 2021/09/01 06:00
CRDT- 2021/07/07 20:10
PHST- 2020/09/28 00:00 [received]
PHST- 2021/04/29 00:00 [revised]
PHST- 2021/06/25 00:00 [accepted]
PHST- 2021/07/08 06:00 [pubmed]
PHST- 2021/09/01 06:00 [medline]
PHST- 2021/07/07 20:10 [entrez]
AID - S1438-4221(21)00046-1 [pii]
AID - 10.1016/j.ijmm.2021.151517 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2021 Aug;311(6):151517. doi: 10.1016/j.ijmm.2021.151517. 
      Epub 2021 Jun 30.