PMID- 34233852
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220301
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 121
IP  - 3
DP  - 2022 Mar
TI  - Characterization of the stemness and osteogenic potential of oral and sinus 
      mucosal cells.
PG  - 652-659
LID - S0929-6646(21)00297-7 [pii]
LID - 10.1016/j.jfma.2021.06.017 [doi]
AB  - BACKGROUND/PURPOSE: Covering the wounds from guided bone regeneration and sinus 
      floor elevation with oral and sinus mucosa is a fundamental criterion for 
      success. This study aimed to verify the regeneration capability of the mucosal 
      connective tissue stromal cells by characterizing their stemness and osteogenic 
      potentials. METHODS: Bone marrow stromal cells (BMSCs), alveolar mucosa cells 
      (AMCs), keratinized gingival cells (KGCs), and sinus mucosal cells (SMCs), were 
      isolated from four Sprague-Dawley rats. The morphology and viability of the cells 
      were investigated under a confocal microscope and by Alamar Blue. Stem cell 
      surface markers were evaluated by flow cytometry. Expressions of pluripotent 
      factors after initial seeding and an early osteogenic gene following 24 h of 
      osteoinduction were evaluated by realtime PCR. Trilineage differentiation 
      capability in long-term inductive cell culture was assessed by Alizarin Red, 
      Alcian Blue, and Oil Red O staining. RESULTS: BMSCs and AMCs were larger cells 
      with smaller aspect ratios relative to KGCs and SMCs, and BMSCs revealed the 
      greatest initial viability but the slowest proliferation. More than 94% of BMSCs, 
      AMCs, and KGCs were double-positive for CD73 and CD90. Compared with BMSCs, AMCs 
      expressed significantly higher Oct4 but reduced Cbfa1 after initial seeding, and 
      AMCs and SMCs expressed significantly higher Cbfa1 following 24 h of 
      osteoinduction. In long-term inductive cell culture, osteogenesis was observed in 
      BMSCs, AMCs, and SMCs, chondrogenesis was observed in BMSCs, AMCs, and KGCs, and 
      adipogenesis was evident in only BMSCs. CONCLUSION: AMCs contain a high 
      percentage of stem/progenitor cells and show differentiation capability toward 
      osteogenic lineage.
CI  - Copyright (c) 2021 Formosan Medical Association. Published by Elsevier B.V. All 
      rights reserved.
FAU - Chen, Ming-Hsu
AU  - Chen MH
AD  - Department of Otorhinolaryngology, Cathay General Hospital, Taipei, Taiwan.
FAU - Tai, Wei-Chiu
AU  - Tai WC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Cheng, Nai-Chen
AU  - Cheng NC
AD  - Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
FAU - Chang, Ching-He
AU  - Chang CH
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Chang, Po-Chun
AU  - Chang PC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan; Division of Periodontics, Department of Dentistry, 
      National Taiwan University Hospital, Taipei, Taiwan; School of Dentistry, College 
      of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic 
      address: changpc@ntu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20210704
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sinus Floor Augmentation
OTO - NOTNLM
OT  - Bone regeneration
OT  - Gingiva
OT  - Mesenchymal stem cells
OT  - Nasal mucosa
COIS- Declaration of competing interest The authors have no conflicts of interest 
      relevant to this article.
EDAT- 2021/07/09 06:00
MHDA- 2022/03/03 06:00
CRDT- 2021/07/08 05:44
PHST- 2021/05/18 00:00 [received]
PHST- 2021/06/10 00:00 [revised]
PHST- 2021/06/17 00:00 [accepted]
PHST- 2021/07/09 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/07/08 05:44 [entrez]
AID - S0929-6646(21)00297-7 [pii]
AID - 10.1016/j.jfma.2021.06.017 [doi]
PST - ppublish
SO  - J Formos Med Assoc. 2022 Mar;121(3):652-659. doi: 10.1016/j.jfma.2021.06.017. 
      Epub 2021 Jul 4.