PMID- 3423741
OWN - NLM
STAT- MEDLINE
DCOM- 19880205
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 26
IP  - 6
DP  - 1987 Dec
TI  - Semi-purification of an immunosuppressor substance secreted by Streptococcus 
      mutans that plays a role in the protection of the bacteria in the host.
PG  - 755-61
AB  - Crude extracellular products of Streptococcus mutans (CEP-Sm) suppress the 
      proliferative response to phytohaemagglutinin of human peripheral blood 
      mononuclear cells and the primary immune response of C57BL/6 mice to sheep 
      erythrocytes. This immunosuppressive effect favours the survival of the 
      microorganism, and the bacteria lose the ability to secrete immunosuppressor 
      substance if previously subcultured several times. Cells incubated with CEP-Sm 
      and subsequently washed recover the ability to proliferate. Traces of CEP-Sm or 
      to short a time of contact between CEP-Sm and the target immune system induced 
      higher proliferative ratios or higher in vivo immune responses than controls, 
      respectively. The proliferative values of cultures supplemented with CEP-Sm were 
      parallel to the control values up to a certain time, after which they dropped 
      abruptly. This drop is followed by a proliferation, and the higher the amount of 
      CEP-Sm added to the cultures, the shorter the time until the proliferation. 
      CEP-Sm was fractionated by means of ion exchange chromatography followed by 
      double preparative isoelectrofocusing, ending in a subfraction of isoelectric 
      point between 3.9 and 4.2, containing a heat-unstable material stainable by 
      Coomassie blue but unstainable by periodic acid Schiff or methylene blue, and 
      having a maximum optical density of 280 nm.
FAU - Santarem, M M
AU  - Santarem MM
AD  - Department of Immunology, Institute for Biomedical Sciences Abel Salazar, Porto, 
      Portugal.
FAU - Porto, M T
AU  - Porto MT
FAU - Ferreira, P
AU  - Ferreira P
FAU - Soares, R
AU  - Soares R
FAU - Arala-Chaves, M P
AU  - Arala-Chaves MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Immunosuppressive Agents/*isolation & purification/pharmacology
MH  - Leukocytes, Mononuclear/*drug effects
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phytohemagglutinins/pharmacology
MH  - Streptococcus mutans/*analysis
EDAT- 1987/12/01 00:00
MHDA- 1987/12/01 00:01
CRDT- 1987/12/01 00:00
PHST- 1987/12/01 00:00 [pubmed]
PHST- 1987/12/01 00:01 [medline]
PHST- 1987/12/01 00:00 [entrez]
AID - 10.1111/j.1365-3083.1987.tb02313.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1987 Dec;26(6):755-61. doi: 10.1111/j.1365-3083.1987.tb02313.x.