PMID- 34240224
OWN - NLM
STAT- MEDLINE
DCOM- 20210913
LR  - 20240226
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 23
IP  - 5
DP  - 2021 May
TI  - Therapeutic effects of quinine in a mouse model of atopic dermatitis.
LID - 313 [pii]
LID - 10.3892/mmr.2021.11952 [doi]
AB  - Atopic dermatitis (AD) is a chronic inflammatory skin disease that seriously 
      affects quality of life. Quinine is a bitter taste receptor agonist that exhibits 
      antimalarial effects. The aim of the present study was to examine the therapeutic 
      effects of quinine in AD‑like mice. AD was induced with 2,4‑dinitrochlorobenzene, 
      and the mice were treated with 10 mg/kg quinine for 1, 4 and 7 days. A total of 
      60 BALB/c mice were divided into the following groups: Healthy, AD‑like, AD‑like 
      + quinine and healthy + quinine, with 1, 4 and 7 days groups for each treatment. 
      Blood was extracted from all mice and ELISA was performed to detect 
      immunoglobulin E (IgE) levels. H&E‑stained tissue sections were prepared from 
      skin lesions on the backs of the mice and pathological changes were observed. 
      Cytokines were detected via ELISA, and the filaggrin (FLG) and kallikrein‑7 
      (KLK7) proteins were detected via western blotting and immunohistochemistry. IKKalpha 
      and NF‑kappaB mRNA were analyzed via reverse transcription‑quantitative PCR. Quinine 
      ameliorated skin damage in the AD‑like mice, reduced IgE expression in the blood, 
      inhibited expression of IKKalpha and NF‑kappaB, reduced cytokine secretion, reduced KLK7 
      expression, reduced scratching frequency, increased FLG expression and repaired 
      the skin barrier. These results suggested that quinine exhibited therapeutic 
      effects in AD‑like mice.
FAU - Zhang, Qian
AU  - Zhang Q
AD  - Department of Dermatology, Shenzhen University General Hospital, Shenzhen 
      University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Jiang, Hongjing
AU  - Jiang H
AD  - Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, P.R. 
      China.
FAU - Liu, Miao
AU  - Liu M
AD  - School of Food and Biological Engineering, Shaanxi University of Science and 
      Technology, Xian, Shaanxi 710021, P.R. China.
FAU - Li, Xinchen
AU  - Li X
AD  - School of Food and Biological Engineering, Shaanxi University of Science and 
      Technology, Xian, Shaanxi 710021, P.R. China.
FAU - Zhou, Murong
AU  - Zhou M
AD  - College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 
      Guangdong 518060, P.R. China.
FAU - Lyu, Yansi
AU  - Lyu Y
AD  - Department of Dermatology, Shenzhen University General Hospital, Shenzhen 
      University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Huang, Jingkai
AU  - Huang J
AD  - Department of Dermatology, Shenzhen University General Hospital, Shenzhen 
      University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Chen, Si
AU  - Chen S
AD  - Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, P.R. 
      China.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Dermatology, Shenzhen University General Hospital, Shenzhen 
      University, Shenzhen, Guangdong 518060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210709
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (NF-kappa B)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - A7V27PHC7A (Quinine)
RN  - EC 2.7.10.1 (Fgfr1 protein, mouse)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 2.7.11.10 (Chuk protein, mouse)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 3.4.21.- (Kallikreins)
RN  - EC 3.4.21.- (Klk7 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - Dermatitis, Atopic/chemically induced/*drug therapy/metabolism/pathology
MH  - Dinitrochlorobenzene/toxicity
MH  - Disease Models, Animal
MH  - I-kappa B Kinase/genetics/metabolism
MH  - Immunoglobulin E/blood
MH  - Kallikreins/genetics/metabolism
MH  - Male
MH  - Mice, Inbred BALB C
MH  - NF-KappaB Inhibitor alpha/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Quinine/*pharmacology/*therapeutic use
MH  - Receptor, Fibroblast Growth Factor, Type 1/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - Skin/drug effects/pathology
MH  - Mice
PMC - PMC7974254
OTO - NOTNLM
OT  - NF‑kappaB signaling pathway
OT  - atopic dermatitis
OT  - quinine
COIS- The authors declare that they have no competing interests.
EDAT- 2021/07/10 06:00
MHDA- 2021/09/14 06:00
PMCR- 2021/03/02
CRDT- 2021/07/09 07:10
PHST- 2020/07/06 00:00 [received]
PHST- 2021/01/21 00:00 [accepted]
PHST- 2021/07/09 07:10 [entrez]
PHST- 2021/07/10 06:00 [pubmed]
PHST- 2021/09/14 06:00 [medline]
PHST- 2021/03/02 00:00 [pmc-release]
AID - 313 [pii]
AID - MMR-0-0-11952 [pii]
AID - 10.3892/mmr.2021.11952 [doi]
PST - ppublish
SO  - Mol Med Rep. 2021 May;23(5):313. doi: 10.3892/mmr.2021.11952. Epub 2021 Jul 9.