PMID- 34241828
OWN - NLM
STAT- MEDLINE
DCOM- 20211214
LR  - 20220716
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 52
IP  - 4
DP  - 2021 Dec
TI  - Development of a rapid loop-mediated isothermal amplification (LAMP) assay for 
      visual detection of porcine parvovirus (PPV) and its application.
PG  - 1725-1732
LID - 10.1007/s42770-021-00569-1 [doi]
AB  - Porcine parvovirus (PPV) infection is one of the most important causes of 
      reproductive failure in pigs impacting the piggery industry globally with huge 
      economic losses. A cost-effective, simple, rapid, specific, and sensitive method 
      is critical for monitoring PPV infection on pig farms. The main aim of the 
      present study was to develop and evaluate a loop-mediated isothermal 
      amplification (LAMP) assay for rapid visual detection of porcine parvovirus (PPV) 
      in pigs. A set of six LAMP primers including two outer primers, two inner 
      primers, and two loop primers were designed utilizing the conserved region of 
      capsid protein VP2 gene sequences of PPV and was applied for detection of PPV 
      from porcine samples. Time and temperature conditions for amplification of PPV 
      genes were optimized to be 30 min at 63  degrees C. The developed assay was ten-fold more 
      sensitive than conventional PCR with analytical sensitivity of 20 pg and 200 pg, 
      respectively. This is the first report of detection of PPV by LAMP assay from 
      India. The assay did not cross-react with porcine circovirus type 2 (PCV2), 
      porcine reproductive and respiratory syndrome virus (PRRSV), or classical swine 
      fever virus (CSFV). The LAMP assay was assembled into a LAMP assay kit of 20 
      reactions and was validated in different laboratories in India. The newly 
      developed LAMP assay was proved to be a specific, sensitive, rapid, and simple 
      method for visual detection of PPV which does not require even costly equipments 
      for performing the test. It complements and extends previous methods for PPV 
      detection and provides an alternative approach for detection of PPV.
CI  - (c) 2021. Sociedade Brasileira de Microbiologia.
FAU - Rajkhowa, S
AU  - Rajkhowa S
AD  - Animal Health Laboratory, Indian Council of Agricultural Research - National 
      Research Centre On Pig (ICAR-NRC On Pig), Rani, Guwahati, 781131, Assam, India. 
      swaraj.rajkhowa@gmail.com.
FAU - Choudhury, M
AU  - Choudhury M
AD  - Animal Health Laboratory, Indian Council of Agricultural Research - National 
      Research Centre On Pig (ICAR-NRC On Pig), Rani, Guwahati, 781131, Assam, India.
FAU - Pegu, S R
AU  - Pegu SR
AD  - Animal Health Laboratory, Indian Council of Agricultural Research - National 
      Research Centre On Pig (ICAR-NRC On Pig), Rani, Guwahati, 781131, Assam, India.
FAU - Sarma, D K
AU  - Sarma DK
AD  - Department of Microbiology, AAU, Guwahati, India.
FAU - Hussain, I
AU  - Hussain I
AD  - Division of Veterinary Microbiology & Immunology, SKUAST-Kashmir, Srinagar, 
      India.
LA  - eng
GR  - DBT-NER/LIVS/11/2012/Department of Biotechnology , Ministry of Science and 
      Technology/
PT  - Journal Article
DEP - 20210709
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
RN  - LAMP assay
SB  - IM
MH  - Animals
MH  - *Molecular Diagnostic Techniques
MH  - *Nucleic Acid Amplification Techniques
MH  - *Parvoviridae Infections/diagnosis/veterinary
MH  - *Parvovirus, Porcine/genetics
MH  - Sensitivity and Specificity
MH  - Swine
MH  - *Swine Diseases/diagnosis
PMC - PMC8578513
OTO - NOTNLM
OT  - Loop-mediated isothermal amplification. Porcine parvovirus. Sensitivity. 
      Specificity. Visual detection
COIS- The authors declare no competing interests.
EDAT- 2021/07/10 06:00
MHDA- 2021/12/15 06:00
PMCR- 2022/07/09
CRDT- 2021/07/09 12:26
PHST- 2020/12/25 00:00 [received]
PHST- 2021/07/01 00:00 [accepted]
PHST- 2021/07/10 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/07/09 12:26 [entrez]
PHST- 2022/07/09 00:00 [pmc-release]
AID - 10.1007/s42770-021-00569-1 [pii]
AID - 569 [pii]
AID - 10.1007/s42770-021-00569-1 [doi]
PST - ppublish
SO  - Braz J Microbiol. 2021 Dec;52(4):1725-1732. doi: 10.1007/s42770-021-00569-1. Epub 
      2021 Jul 9.