PMID- 34245860
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220531
IS  - 1873-3913 (Electronic)
IS  - 0898-6568 (Linking)
VI  - 86
DP  - 2021 Oct
TI  - Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via 
      miR-196b-mediated OSMR regulation.
PG  - 110077
LID - S0898-6568(21)00166-2 [pii]
LID - 10.1016/j.cellsig.2021.110077 [doi]
AB  - Cardiac hypertrophy is the uppermost risk factor for the development of heart 
      failure, leading to irreversible cardiac structural remodeling and sudden death. 
      As a major mediator of cardiac remodeling, oncostatin M (OSM) and its receptor, 
      OSMR, attract plenty of interest. Recent studies have demonstrated key effects of 
      noncoding RNAs on myocardial remodeling. However, whether noncoding RNAs that 
      regulate the expression of OSMR would regulate the process of remodeling remain 
      unclear. Herein, we observed that long noncoding RNA (lncRNA) Pvt1 expression 
      showed to be significantly elicited by aortic banding (AB) operation in vivo and 
      by angiotensin (Ang II) treatment in vitro. Pvt1 knockdown significantly 
      attenuated the myocardial hypertrophy caused by pressure overload within rats and 
      the cardiac myocyte hypertrophy caused by Ang II in vitro. Moreover, Pvt1 
      knockdown also decreased cellular myomesin and B-raf, which was involved in OSM 
      function in cardiac remodeling. Based on online tools prediction, miR-196b may 
      simultaneously target Pvt1 and OSMR 3' untranslated region (UTR). In rat H9c2 
      cells and primary cardiac myocyte, Pvt1 and miR-196b exerted negative regulatory 
      effects on each other and miR-196b negatively regulated OSMR expression. Pvt1 
      directly targeted miR-196b to relieve miR-196b-induced OSMR suppression via 
      acting as a competing endogenous RNA (ceRNA). Moreover, the effect of miR-196b 
      suppression upon the B-raf was opposite to Pvt1 knockdown, and miR-196b 
      suppression might significantly attenuate the effect of Pvt1 knockdown. In 
      summary, Pvt1/miR-196b axis modulating cardiomyocyte hypertrophy and remodeling 
      via OSMR. Our findings provide a rationale for further studies on the potential 
      therapeutic benefits of Pvt1 function and mechanism in cardiac and cardiomyocyte 
      hypertrophy by a lncRNA-miRNA-mRNA network.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Wu, Qingqing
AU  - Wu Q
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular 
      Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 
      430060, China.
FAU - Chen, Qiuxiang
AU  - Chen Q
AD  - Renmin Hospital of Wuhan University, Department of Neurology, Wuhan 430060, 
      China.
FAU - Wang, Juan
AU  - Wang J
AD  - The Fifth Affiliated Hospital of Xin Jiang medical University, Department of 
      Cardiology, Urumchi 830001, China.
FAU - Fan, Di
AU  - Fan D
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular 
      Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 
      430060, China.
FAU - Zhou, Heng
AU  - Zhou H
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular 
      Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 
      430060, China.
FAU - Yuan, Yuan
AU  - Yuan Y
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular 
      Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 
      430060, China.
FAU - Shen, Difei
AU  - Shen D
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular 
      Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 
      430060, China; The Fifth Affiliated Hospital of Xin Jiang medical University, 
      Department of Cardiology, Urumchi 830001, China. Electronic address: 
      rm000137@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210708
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - Cardiomegaly/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - Myocytes, Cardiac/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Rats
OTO - NOTNLM
OT  - Cardiomyocyte hypertrophy
OT  - Cardiomyocyte remodeling
OT  - OSMR
OT  - Pvt1
OT  - miR-196b
EDAT- 2021/07/11 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/07/10 20:10
PHST- 2021/02/09 00:00 [received]
PHST- 2021/06/30 00:00 [revised]
PHST- 2021/07/05 00:00 [accepted]
PHST- 2021/07/11 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/07/10 20:10 [entrez]
AID - S0898-6568(21)00166-2 [pii]
AID - 10.1016/j.cellsig.2021.110077 [doi]
PST - ppublish
SO  - Cell Signal. 2021 Oct;86:110077. doi: 10.1016/j.cellsig.2021.110077. Epub 2021 
      Jul 8.