PMID- 34249264
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 24
IP  - 5
DP  - 2021 May
TI  - Quercetin protects islet beta-cells from oxidation-induced apoptosis via Sirt3 in 
      T2DM.
PG  - 629-635
LID - 10.22038/ijbms.2021.52005.11792 [doi]
AB  - OBJECTIVES: Sirt3 may regulate ROS production and might be involved in beta-cell 
      apoptosis, which plays an important role in the progression of type 2 diabetes 
      mellitus (T2DM). Quercetin is a potent anti-oxidative bioflavonoid, but its 
      effects on T2DM remain to be explored. This study aimed to investigate the 
      effects of quercetin on beta-cell apoptosis and explore its mechanisms. MATERIALS 
      AND METHODS: The effects of quercetin were conducted on db/db mice and INS1 
      cells. Fasting blood glucose was determined by the colorimetric method, serum 
      insulin was measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, 
      Sirt3 in INS1 cells was knocked down by plasmid transfection. The antioxidant 
      proteins (SOD2 and CAT), apoptosis proteins (cleaved Caspase-3, Bax, and BCL-2), 
      and Sirt3 protein in pancreases and INS1 cells were determined by western 
      blotting. RESULTS: When INS1 cells and diabetic mice were treated with quercetin, 
      the levels of SOD2, CAT, and Sirt3 proteins were increased, the levels of cleaved 
      Caspase-3 and the ratio of Bax to BCL-2 were decreased at different degrees, 
      along with reduced blood glucose levels and elevated insulin levels in diabetic 
      mice. When Sirt3 was knocked down in INS1 cells, increase of two antioxidants and 
      decrease of cell apoptosis generated by quercetin could not occur. CONCLUSION: 
      Quercetin protected islet beta-cells from oxidation-induced apoptosis via Sirt3 in 
      T2DM, which would be beneficial to develop new strategies for preventing beta-cell 
      failure in T2DM.
FAU - Wang, Jian-Yun
AU  - Wang JY
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
FAU - Nie, Ya-Xing
AU  - Nie YX
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
FAU - Dong, Bing-Zheng
AU  - Dong BZ
AD  - Department of Urology, Xuzhou Central Hospital, The Affiliated School of Clinical 
      Medicine of Xuzhou Medical University, Xuzhou 221009, Jiangsu, PR China.
FAU - Cai, Zhi-Chen
AU  - Cai ZC
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
FAU - Zeng, Xuan-Kai
AU  - Zeng XK
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
AD  - Xuzhou Jiasheng Pharmaceutical Technology Co., Ltd., Xuzhou 221000, Jiangsu, PR 
      China.
FAU - Du, Lei
AU  - Du L
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
FAU - Zhu, Xia
AU  - Zhu X
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
FAU - Yin, Xiao-Xing
AU  - Yin XX
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou 221002, Jiangsu, PR China.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC8244598
OTO - NOTNLM
OT  - Apoptosis
OT  - Oxidative stress
OT  - Quercetin
OT  - Sirt3
OT  - beta-cell
COIS- The authors declare that there are no con fl icts of interest associated with this 
      work.
EDAT- 2021/07/13 06:00
MHDA- 2021/07/13 06:01
PMCR- 2021/05/01
CRDT- 2021/07/12 05:51
PHST- 2020/09/16 00:00 [received]
PHST- 2021/04/11 00:00 [accepted]
PHST- 2021/07/12 05:51 [entrez]
PHST- 2021/07/13 06:00 [pubmed]
PHST- 2021/07/13 06:01 [medline]
PHST- 2021/05/01 00:00 [pmc-release]
AID - 10.22038/ijbms.2021.52005.11792 [doi]
PST - ppublish
SO  - Iran J Basic Med Sci. 2021 May;24(5):629-635. doi: 
      10.22038/ijbms.2021.52005.11792.