PMID- 34249720
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - ASRGL1 Correlates With Immune Cell Infiltration in Hepatocellular Carcinoma and 
      Can Serve as a Prognostic Biomarker.
PG  - 680070
LID - 10.3389/fonc.2021.680070 [doi]
LID - 680070
AB  - BACKGROUND: The enzyme L-asparaginase (ASRGL1) catalyzes the hydrolysis of 
      L-asparagine (Asn) to L-aspartic acid (Asp) and ammonia. Numerous studies have 
      shown a strong correlation between ASRGL1 expression and tumorigenesis. However, 
      the expression and biological function of ASRGL1 in hepatocellular carcinoma 
      (HCC) are still unclear. METHODS: We explored the mRNA expression of ASRGL1 in 
      HCC using the HCCDB, Oncomine, and TIMER 2.0 databases. Western blotting and 
      immunohistochemical analyses were also used to determine the mRNA expression of 
      ASRGL1 in HCC. LinkedOmics was used to analyze the genes co-expressed with ASRGL1 
      and regulators including kinases, miRNAs, and transcription factors. The Gene 
      Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways 
      of the co-expressed genes were also investigated using LinkedOmics. The 
      correlation between ASRGL1 expression and immune infiltrates was analyzed using 
      the TIMER 2.0 and Gene Expression Profiling Interactive Analysis (GEPIA) 
      databases. The effects of ASRGL1 expression on patient outcome were investigated 
      using the UALCAN and GEPIA databases, and the Kaplan-Meier plotter. c-Bioportal 
      was used to explore the mutations of ASRGL1 in HCC. RESULTS: Compared with the 
      adjacent tissues, ASRGL1 was upregulated in HCC. High ASRGL1 expression in HCC 
      indicated poor relapse-free survival, progression-free survival, disease-specific 
      survival, and overall survival. The expression of ASRGL1 was significantly 
      correlated with infiltrating levels of B cells, CD4+ T cells, macrophages, 
      neutrophils, and dendritic cells in HCC. CONCLUSION: Our findings suggest that 
      ASRGL1 is overexpressed in HCC and that ASRGL1 expression was significantly 
      correlated with immune infiltration in HCC and prognosis. Therefore, ASRGL1 may 
      serve as a biomarker for the early diagnosis and treatment of HCC.
CI  - Copyright (c) 2021 Xue, Gao, Cui, Zhang, Lei, Li, Zhu and Qin.
FAU - Xue, Cailin
AU  - Xue C
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Gao, Peng
AU  - Gao P
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Cui, Xiaohan
AU  - Cui X
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Zhang, Xudong
AU  - Zhang X
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Lei, Jin
AU  - Lei J
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Li, Renzhi
AU  - Li R
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Zhu, Chunfu
AU  - Zhu C
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
FAU - Qin, Xihu
AU  - Qin X
AD  - Department of Hepatobiliary Surgery, The Affiliated Changzhou No. 2 People's 
      Hospital of Nanjing Medical University, Changzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210625
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8267417
OTO - NOTNLM
OT  - ASRGL1
OT  - HCC
OT  - biomarker
OT  - immune
OT  - immune cell infiltration
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/13 06:00
MHDA- 2021/07/13 06:01
PMCR- 2021/01/01
CRDT- 2021/07/12 05:56
PHST- 2021/03/26 00:00 [received]
PHST- 2021/05/31 00:00 [accepted]
PHST- 2021/07/12 05:56 [entrez]
PHST- 2021/07/13 06:00 [pubmed]
PHST- 2021/07/13 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.680070 [doi]
PST - epublish
SO  - Front Oncol. 2021 Jun 25;11:680070. doi: 10.3389/fonc.2021.680070. eCollection 
      2021.