PMID- 34251294
OWN - NLM
STAT- MEDLINE
DCOM- 20220418
LR  - 20230828
IS  - 2047-7163 (Electronic)
IS  - 0036-8504 (Print)
IS  - 0036-8504 (Linking)
VI  - 104
IP  - 3
DP  - 2021 Jul-Sep
TI  - RNA sequencing identified novel target genes for Adansonia digitata in breast and 
      colon cancer cells.
PG  - 368504211032084
LID - 10.1177/00368504211032084 [doi]
LID - 00368504211032084
AB  - Adansonia digitata exhibits numerous beneficial effects. In the current study, we 
      investigated the anti-cancer effects of four different extracts of A. digitata 
      (polar and non-polar extracts of fruit powder and fibers) on the proliferation of 
      human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian 
      cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of 
      the effective A. digitata fraction on the gene expression profiles of responsive 
      cells. The results indicated that only the polar extract of the A. digitata 
      fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, 
      but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted 
      in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. 
      We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 
      (CSNK2) isoform in HCT116 cells and report, for the first time, the potential 
      involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. 
      Together, these findings provide evidence supporting the anti-cancer potential of 
      the polar extract of A. digitata fibers in this experimental model of breast and 
      colon cancers.
FAU - El-Masry, Omar S
AU  - El-Masry OS
AUID- ORCID: 0000-0002-0226-2923
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
FAU - Goja, Arafat
AU  - Goja A
AD  - Department of Clinical Nutrition, College of Applied Medical Sciences, Imam 
      Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
FAU - Rateb, Mostafa
AU  - Rateb M
AD  - School of Computing, Engineering & Physical Sciences, University of the West of 
      Scotland, Paisley, UK.
AD  - Marine Biodiscovery Centre, School of Natural & Computing Sciences, University of 
      Aberdeen, Aberdeen, UK.
FAU - Owaidah, Amani Y
AU  - Owaidah AY
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
FAU - Alsamman, Khaldoon
AU  - Alsamman K
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Sci Prog
JT  - Science progress
JID - 0411361
RN  - 0 (Plant Extracts)
RN  - EC 2.7.11.1 (Casein Kinase II)
SB  - IM
MH  - *Adansonia
MH  - Apoptosis
MH  - Casein Kinase II
MH  - Cell Line, Tumor
MH  - *Colonic Neoplasms/drug therapy/genetics
MH  - Female
MH  - Humans
MH  - *Ovarian Neoplasms
MH  - Plant Extracts
MH  - Sequence Analysis, RNA
PMC - PMC10450698
OTO - NOTNLM
OT  - Adansonia digitata
OT  - HCT-116
OT  - LC-HRMS
OT  - MCF-7
OT  - RNA sequencing
OT  - cell proliferation
OT  - colon cancer
EDAT- 2021/07/13 06:00
MHDA- 2022/04/19 06:00
PMCR- 2021/07/12
CRDT- 2021/07/12 12:21
PHST- 2021/07/12 12:21 [entrez]
PHST- 2021/07/13 06:00 [pubmed]
PHST- 2022/04/19 06:00 [medline]
PHST- 2021/07/12 00:00 [pmc-release]
AID - 10.1177_00368504211032084 [pii]
AID - 10.1177/00368504211032084 [doi]
PST - ppublish
SO  - Sci Prog. 2021 Jul-Sep;104(3):368504211032084. doi: 10.1177/00368504211032084.