PMID- 34254463
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20211028
IS  - 2471-5646 (Electronic)
IS  - 2471-5638 (Linking)
VI  - 335
IP  - 7
DP  - 2021 Aug
TI  - Time-course changes in the regulation of ions and amino acids in the hard clam 
      Meretrix lusoria upon lower salinity challenge.
PG  - 602-613
LID - 10.1002/jez.2503 [doi]
AB  - In this study, we examined ion and amino acid regulation in the gill and mantle 
      of the hard clam Meretrix lusoria. We found that the osmolality and Na(+) and 
      Cl(-) concentrations of hard clam hemolymph were significantly reduced after 
      transferring clams from the salinity of their natural habitat [20 per thousand saltwater 
      (SW)] to a lower salinity environment (10 per thousand SW). Specific activities of Na(+) , 
      K(+) -ATPase (NKA), which provides the driving force for the secondary ion 
      transport associated with cell osmoregulation in gills and mantles, were 
      unaffected during the acclimation to lower salinity. In contrast, there was a 
      significant decline in the contents of free amino acids (FAAs) in the gills and 
      mantles of hard clams during lower salinity acclimation. Taurine was established 
      to be the dominant FAA, the content of which is considerably higher than that of 
      other FAAs in the hard clam. Following acclimation to the lower salinity 
      environment, mRNA expression of the taurine transporter (TAUT), which plays a 
      pivotal role in regulating intracellular taurine contents, was significantly 
      upregulated in the gill and downregulated in the mantle of hard clams at 
      different time points. However, the relative abundance of TAUT protein in the 
      gill and mantle was significantly increased after transfer from 20 per thousand SW to 10 per thousand SW, 
      which may reflect feedback regulation in response to reduced taurine contents in 
      the gill and mantle of hard clams. Collectively, the findings of this study 
      provide important insights on the dynamic processes of ion and amino acid 
      regulation in the peripheral tissues of bivalves.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Lin, Chia-Hao
AU  - Lin CH
AD  - Department of Marine Biotechnology, National Kaohsiung University of Science and 
      Technology, Kaohsiung, Taiwan.
AD  - The iEGG and Animal Biotechnology Center, National Chung Hsing University, 
      Taichung, Taiwan.
FAU - Yeh, Po-Ling
AU  - Yeh PL
AD  - Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
FAU - Lee, Tsung-Han
AU  - Lee TH
AUID- ORCID: 0000-0002-3542-4335
AD  - The iEGG and Animal Biotechnology Center, National Chung Hsing University, 
      Taichung, Taiwan.
AD  - Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210712
PL  - United States
TA  - J Exp Zool A Ecol Integr Physiol
JT  - Journal of experimental zoology. Part A, Ecological and integrative physiology
JID - 101710204
RN  - 0 (Amino Acids)
RN  - 0 (Ions)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Amino Acids
MH  - Animals
MH  - *Bivalvia
MH  - Ions
MH  - *Salinity
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
OTO - NOTNLM
OT  - K+-ATPase
OT  - Na+
OT  - free amino acid
OT  - hard clam
OT  - osmoregulation
OT  - taurine transporter
EDAT- 2021/07/14 06:00
MHDA- 2021/10/29 06:00
CRDT- 2021/07/13 06:54
PHST- 2021/04/27 00:00 [revised]
PHST- 2020/11/11 00:00 [received]
PHST- 2021/06/16 00:00 [accepted]
PHST- 2021/07/14 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
PHST- 2021/07/13 06:54 [entrez]
AID - 10.1002/jez.2503 [doi]
PST - ppublish
SO  - J Exp Zool A Ecol Integr Physiol. 2021 Aug;335(7):602-613. doi: 10.1002/jez.2503. 
      Epub 2021 Jul 12.