PMID- 34256037
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20211015
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 478
DP  - 2021 Oct
TI  - The nephric mesenchyme lineage of intermediate mesoderm is derived from 
      Tbx6-expressing derivatives of neuro-mesodermal progenitors via BMP-dependent 
      Osr1 function.
PG  - 155-162
LID - S0012-1606(21)00169-X [pii]
LID - 10.1016/j.ydbio.2021.07.006 [doi]
AB  - In vertebrate embryos, the kidney primordium metanephros is formed from two 
      distinct cell lineages, Wolffian duct and metanephric mesenchyme, which were 
      classically grouped as intermediate mesoderm. Whereas the reciprocal interactions 
      between these two cell populations in kidney development have been studied 
      extensively, the mechanisms generating them remain elusive. Here, we show that 
      the mouse cell lineage that forms nephric mesenchyme develops as a subpopulation 
      of Tbx6-expressing mesodermal precursor derivatives of neuro-mesodermal 
      progenitors (NMPs) under the condition of bone morphogenetic protein 
      (BMP)-signal-dependent Osr1 expression. The Osr1-expressing nephric mesenchyme 
      precursors were confirmed as descendants of NMPs because they were labeled by 
      Sox2 N1 enhancer-EGFP. In Tbx6 mutant embryos, nephric mesenchyme changed its 
      fate into neural tissues, which reflected its NMP origin. In Osr1 mutant embryos, 
      the specific region of the Tbx6-expressing mesoderm precursor, which normally 
      expresses Osr1 and develops into the nephric mesenchyme, instead expressed the 
      somite marker FoxC2. BMP signaling activated Osr1 expression in a region of 
      TBX6-expressing mesoderm and elicited nephric mesenchyme development. This study 
      suggested a new model of cell lineage segregation during gastrulation.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Hayashi, Shinichi
AU  - Hayashi S
AD  - Laboratory of Embryology, Institute of Medical Advanced Sciences, Tokushima 
      University, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan.
FAU - Suzuki, Hitomi
AU  - Suzuki H
AD  - Laboratory of Embryology, Institute of Medical Advanced Sciences, Tokushima 
      University, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan.
FAU - Takemoto, Tatsuya
AU  - Takemoto T
AD  - Laboratory of Embryology, Institute of Medical Advanced Sciences, Tokushima 
      University, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan. Electronic address: 
      takemoto.tatsuya@tokushima-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210710
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Osr1 protein, mouse)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Tbx6 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (mesenchyme fork head 1 protein)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Cell Lineage
MH  - Forkhead Transcription Factors/metabolism
MH  - *Gastrulation
MH  - Kidney/*embryology
MH  - Mesenchymal Stem Cells/physiology
MH  - Mesoderm/cytology/*embryology
MH  - Mice
MH  - Neural Stem Cells/physiology
MH  - Organogenesis
MH  - Signal Transduction
MH  - Somites/cytology/physiology
MH  - Stem Cells/*physiology
MH  - T-Box Domain Proteins/*metabolism
MH  - Transcription Factors/*metabolism
OTO - NOTNLM
OT  - Gastrulation
OT  - Nephric mesenchyme
OT  - Neuro-mesodermal progenitors
COIS- Declaration of competing interest The authors declare no competing financial 
      interests.
EDAT- 2021/07/14 06:00
MHDA- 2021/10/16 06:00
CRDT- 2021/07/13 20:09
PHST- 2021/01/27 00:00 [received]
PHST- 2021/06/07 00:00 [revised]
PHST- 2021/07/07 00:00 [accepted]
PHST- 2021/07/14 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2021/07/13 20:09 [entrez]
AID - S0012-1606(21)00169-X [pii]
AID - 10.1016/j.ydbio.2021.07.006 [doi]
PST - ppublish
SO  - Dev Biol. 2021 Oct;478:155-162. doi: 10.1016/j.ydbio.2021.07.006. Epub 2021 Jul 
      10.