PMID- 34256043
OWN - NLM
STAT- MEDLINE
DCOM- 20210913
LR  - 20210913
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 283
DP  - 2021 Oct 15
TI  - Induction of ischemic tolerance by remote perconditioning or postconditioning as 
      neuroprotective strategy for spinal cord motor neurons.
PG  - 119789
LID - S0024-3205(21)00775-X [pii]
LID - 10.1016/j.lfs.2021.119789 [doi]
AB  - AIMS: The study is focused on the investigation of the mechanisms leading to 
      ischemic tolerance acquisition in the spinal cord neurons via application of 
      non-invasive method of remote conditioning. MATERIAL AND METHODS: We have 
      verified the possibility of neuroprotection of spinal cord in rabbit by using 
      remote perconditioning (PerC) applied during last 12 min of spinal cord ischemia 
      (SC-ischemia) or postconditioning (PostC) applied after 1st (early) or 3rd (late) 
      h of reperfusion. Spinal cord ischemia was induced by occlusion of the aorta 
      below the left renal artery for 20 min. Reperfusion period was 24 or 72 h. Remote 
      conditioning was induced by compression of left forelimb with a tourniquet in 
      3 cycles of 2 min of ischemia, each followed by 2 min of reperfusion. Damaged 
      neurons were detected by Fluoro Jade B method and the modified Tarlov score was 
      used for functional assessment. KEY FINDINGS: The remote conditioning 
      significantly attenuated degeneration of motor neurons in all remote conditioned 
      groups versus both SC-ischemia groups. We detected significant changes in number 
      of Hsp70 positive motor neurons. At 72time point, in the group with remote late 
      PostC we observed significant increase (p < 0.001) of Hsp70 positive motor 
      neurons versus SC- ischemia group and sham control. There was a trend towards 
      improvement of hindlimbs movement. SIGNIFICANCE: This study showed the 
      effectiveness of remote conditioning as a neuroprotective strategy, evidenced by 
      induction of ischemic tolerance leading to decrease of motor neuron degeneration.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Dankova, Marianna
AU  - Dankova M
AD  - Comenius University in Bratislava, Faculty of Medicine, Institute of Histology 
      and Embryology, Sasinkova 4, 811 04 Bratislava, Slovak Republic.
FAU - Domorakova, Iveta
AU  - Domorakova I
AD  - Pavol Jozef Safarik University, Faculty of Medicine, Department of Histology and 
      Embryology, Srobarova 2, 040 01 Kosice, Slovak Republic.
FAU - Fagova, Zuzana
AU  - Fagova Z
AD  - Pavol Jozef Safarik University, Faculty of Medicine, Department of Histology and 
      Embryology, Srobarova 2, 040 01 Kosice, Slovak Republic.
FAU - Stebnicky, Milan
AU  - Stebnicky M
AD  - Pavol Jozef Safarik University, Faculty of Medicine, Department of Histology and 
      Embryology, Srobarova 2, 040 01 Kosice, Slovak Republic; Pavol Jozef Safarik 
      University, Faculty of Medicine, 2nd Department of Surgery and L. Pasteur 
      University Hospital, Rastislavova 43, 040 01 Kosice, Slovak Republic. Electronic 
      address: stebnickymilan@gmail.com.
FAU - Mechirova, Eva
AU  - Mechirova E
AD  - Pavol Jozef Safarik University, Faculty of Medicine, Department of Histology and 
      Embryology, Srobarova 2, 040 01 Kosice, Slovak Republic.
LA  - eng
PT  - Journal Article
DEP - 20210710
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
SB  - IM
MH  - Animals
MH  - *Ischemic Preconditioning
MH  - Male
MH  - Motor Neurons/*metabolism/pathology
MH  - *Neuroprotection
MH  - Rabbits
MH  - Spinal Cord/*metabolism/pathology
MH  - Spinal Cord Ischemia/*metabolism/pathology/*prevention & control
OTO - NOTNLM
OT  - Ischemia
OT  - Rabbit
OT  - Remote perconditioning
OT  - Remote postconditioning
OT  - Spinal cord
EDAT- 2021/07/14 06:00
MHDA- 2021/09/14 06:00
CRDT- 2021/07/13 20:09
PHST- 2020/12/04 00:00 [received]
PHST- 2021/06/17 00:00 [revised]
PHST- 2021/06/28 00:00 [accepted]
PHST- 2021/07/14 06:00 [pubmed]
PHST- 2021/09/14 06:00 [medline]
PHST- 2021/07/13 20:09 [entrez]
AID - S0024-3205(21)00775-X [pii]
AID - 10.1016/j.lfs.2021.119789 [doi]
PST - ppublish
SO  - Life Sci. 2021 Oct 15;283:119789. doi: 10.1016/j.lfs.2021.119789. Epub 2021 Jul 
      10.