PMID- 34256105
OWN - NLM
STAT- MEDLINE
DCOM- 20220112
LR  - 20220112
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 761
DP  - 2021 Sep 14
TI  - Oxandrolone treatment in juvenile rats induced anxiety-like behavior in young 
      adult animals.
PG  - 136104
LID - S0304-3940(21)00482-1 [pii]
LID - 10.1016/j.neulet.2021.136104 [doi]
AB  - AIMS: Oxandrolone (OXA) is a synthetic steroid used for the treatment of clinical 
      conditions associated with catabolic states in humans, including children. 
      However, its behavioral effects are not well known. Our goal was to evaluate the 
      anxiety-like behavior induced in young adult rats after the treatment of juvenile 
      animals with OXA. METHODS: Four-week-old male rats were separated into three 
      groups: Control (CON), therapeutic-like OXA dose (TD), and excessive OXA dose 
      (ED), in which 2.5 and 37.5 mg/kg/day of OXA were administered via gavage for 
      four weeks for TD and ED, respectively. Behavior was evaluated through the 
      elevated plus maze (EPM) and open field (OF) tests. Protein expression of 
      catalase (CAT), superoxide dismutase (SOD), Tumor necrosis factor-alpha (TNF-alpha), and 
      dopamine receptor 2 (DrD2) were analyzed in tissue samples of the hippocampus, 
      amygdala, and prefrontal cortex by Western Blot. RESULTS: OXA induced 
      anxiety-like behaviors in both TD and ED animals; it decreased the time spent in 
      the open arms of the EPM in both groups and reduced the time spent in the central 
      zone of the OF in the TD group. In the hippocampus, CAT expression was higher in 
      TD compared with both control and ED animals. No differences were found in the 
      amygdala and prefrontal cortex. TNF-alpha, SOD, and DrD2 levels were not altered in 
      any of the assessed areas. CONCLUSIONS: Treatment of juvenile rats with OXA led 
      to anxiety-like behavior in young adult animals regardless of the dose used, with 
      minor changes in the antioxidant machinery located in the hippocampus.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Bernardina, Nara Rubia D
AU  - Bernardina NRD
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - de Lima, Randriely Merscher Sobreira
AU  - de Lima RMS
AD  - Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do 
      Sul, Porto Alegre, RS, Brazil.
FAU - Ronchi, Silas N
AU  - Ronchi SN
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - Wan Der Mass, Edgar M
AU  - Wan Der Mass EM
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - Souza, Glauciene J
AU  - Souza GJ
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - Rodrigues, Livia C
AU  - Rodrigues LC
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - Bissoli, Nazare S
AU  - Bissoli NS
AD  - Department of Physiological Sciences, Federal University of Espirito Santo, 
      Vitoria, ES, Brazil.
FAU - Brasil, Girlandia A
AU  - Brasil GA
AD  - Pharmaceutical Sciences Graduate Program, University Vila Velha, Vila Velha, ES, 
      Brazil. Electronic address: dragirlandia@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210710
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Anabolic Agents)
RN  - 0 (Receptors, Dopamine D2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7H6TM3CT4L (Oxandrolone)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Anabolic Agents/administration & dosage/*toxicity
MH  - Animals
MH  - Anxiety/*etiology
MH  - Catalase/metabolism
MH  - Hippocampus/*drug effects/growth & development/metabolism
MH  - Male
MH  - Oxandrolone/administration & dosage/*toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Dopamine D2/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Anxiety
OT  - Behavior
OT  - Juvenile rats
OT  - Oxandrolone
EDAT- 2021/07/14 06:00
MHDA- 2022/01/13 06:00
CRDT- 2021/07/13 20:11
PHST- 2020/09/16 00:00 [received]
PHST- 2021/06/29 00:00 [revised]
PHST- 2021/07/05 00:00 [accepted]
PHST- 2021/07/14 06:00 [pubmed]
PHST- 2022/01/13 06:00 [medline]
PHST- 2021/07/13 20:11 [entrez]
AID - S0304-3940(21)00482-1 [pii]
AID - 10.1016/j.neulet.2021.136104 [doi]
PST - ppublish
SO  - Neurosci Lett. 2021 Sep 14;761:136104. doi: 10.1016/j.neulet.2021.136104. Epub 
      2021 Jul 10.