PMID- 34256876
OWN - NLM
STAT- MEDLINE
DCOM- 20221221
LR  - 20221228
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 127
IP  - 11
DP  - 2022 Jun 14
TI  - High glucose induces apoptosis, glycogen accumulation and suppresses protein 
      synthesis in muscle cells of olive flounder Paralichthys olivaceus.
PG  - 1601-1612
LID - 10.1017/S0007114521002634 [doi]
AB  - The effect and the mechanism of high glucose on fish muscle cells are not fully 
      understood. In the present study, muscle cells of olive flounder (Paralichthys 
      olivaceus) were treated with high glucose (33 mM) in vitro. Cells were incubated 
      in three kinds of medium containing 5 mM glucose, 5 mM glucose and 28 mM mannitol 
      (as an isotonic contrast) or 33 mM glucose named the Control group, the Mannitol 
      group and the high glucose (HG) group, respectively. Results showed that high 
      glucose increased the ADP:ATP ratio and the reactive oxygen species (ROS) level, 
      decreased mitochondrial membrane potential (MMP), induced the release of 
      cytochrome C (CytC) and cell apoptosis. High glucose also led to cell glycogen 
      accumulation by increasing the glucose uptake ability and affecting the mRNA 
      expressions of glycogen synthase and glycogen phosphorylase. Meanwhile, it 
      activated AMP-activated protein kinase (AMPK), inhibited the activity of 
      mammalian target of rapamycin (mTOR) signalling pathway and the expressions of 
      myogenic regulatory factors (MRF). The expressions of myostatin-1 (mstn-1) and E3 
      ubiquitin ligases including muscle RING-finger protein 1 (murf-1) and muscle 
      atrophy F-box protein (mafbx) were also increased by the high glucose treatment. 
      No difference was found between the Mannitol group and the Control group. These 
      results demonstrate that high glucose has the effects of inducing apoptosis, 
      increasing glycogen accumulation and inhibiting protein synthesis on muscle cells 
      of olive flounder. The mitochondria-mediated apoptotic signalling pathway, AMPK 
      and mTOR pathways participated in these biological effects.
FAU - Liu, Jiahuan
AU  - Liu J
AUID- ORCID: 0000-0002-0587-8088
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Pan, Mingzhu
AU  - Pan M
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Huang, Dong
AU  - Huang D
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Wu, Jing
AU  - Wu J
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Liu, Yue
AU  - Liu Y
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Guo, Yanlin
AU  - Guo Y
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Zhang, Wenbing
AU  - Zhang W
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
FAU - Mai, Kangsen
AU  - Mai K
AD  - The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture 
      and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), 
      Ocean University of China, Qingdao266003, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210714
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Flounder/genetics/metabolism
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - Muscle Cells/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Apoptosis
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Cell
OT  - Glucose
OT  - Mitochondria
OT  - Muscle
OT  - Olive flounder
EDAT- 2021/07/15 06:00
MHDA- 2022/12/22 06:00
CRDT- 2021/07/14 05:43
PHST- 2021/07/15 06:00 [pubmed]
PHST- 2022/12/22 06:00 [medline]
PHST- 2021/07/14 05:43 [entrez]
AID - S0007114521002634 [pii]
AID - 10.1017/S0007114521002634 [doi]
PST - ppublish
SO  - Br J Nutr. 2022 Jun 14;127(11):1601-1612. doi: 10.1017/S0007114521002634. Epub 
      2021 Jul 14.