PMID- 34257654
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 1687-8450 (Print)
IS  - 1687-8469 (Electronic)
IS  - 1687-8450 (Linking)
VI  - 2021
DP  - 2021
TI  - Network Pharmacology and Bioinformatics Methods Reveal the Mechanism of 
      Zao-Jiao-Ci in the Treatment of LSCC.
PG  - 8862821
LID - 10.1155/2021/8862821 [doi]
LID - 8862821
AB  - OBJECTIVE: Zao-Jiao-Ci (ZJC), a traditional Chinese medicine, is considered as a 
      promising candidate to treat laryngeal squamous cell carcinoma (LSCC). However, 
      the underlying molecular mechanism remains unclear. METHODS: Gene expression 
      profiles of GSE36668 were available from the GEO database, and differentially 
      expressed genes (DEGs) of LSCC were obtained by R package; subsequently, 
      enrichment analysis on KEGG and GO of DEGs was performed. The active ingredients 
      of ZJC were screened from the TCMSP database, and the matched candidate targets 
      were obtained by PharmMapper. Furthermore, we constructed protein-protein 
      interaction (PPI) networks of DEGs and candidate targets, respectively, and we 
      screened the core network from the merged network through combining the two PPI 
      networks using Cytoscape 3.7.2. The key targets derived from the core network 
      were analyzed to find out the associated KEGG signal enrichment pathway. By the 
      GEPIA online website, Kaplan-Meier analysis was used to complete the overall 
      survival and disease-free survival of the selected genes in the core module. 
      RESULTS: We identified 96 candidate targets of ZJC and 86 DEGs of LSCC, the 
      latter including 50 upregulated genes and 36 downregulated genes. DEGs were 
      obviously enriched in the following biological functions: extracellular structure 
      organization, the extracellular matrix organization, and endodermal cell 
      differentiation. The 60 key targets from the core network were enriched in the 
      signal pathways including transcriptional misregulation cancer, cell cycle, and 
      so on. We found that LSCC patients with high expression of HIST1H3J, HIST1H3F, 
      and ITGA4 had worse overall survival, while higher expression of NTRK1, COPS5, 
      HIST1H3A, and HIST1H3G had significantly worse disease-free survival. CONCLUSION: 
      It suggested that the interaction between ZJC and LSCC was related to the signal 
      pathways of transcriptional misregulation cancer and cell cycle, revealing that 
      it may be the mechanism of ZJC in the treatment of LSCC.
CI  - Copyright (c) 2021 Feng Xiang et al.
FAU - Xiang, Feng
AU  - Xiang F
AUID- ORCID: 0000-0002-3780-020X
AD  - Department of Otolaryngology, YangZhou Hospital of Traditional Chinese Medicine, 
      JiangSu, YangZhou, China.
FAU - Li, Linman
AU  - Li L
AUID- ORCID: 0000-0002-8595-9434
AD  - The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, 
      Guangdong, China.
FAU - Lin, Jieling
AU  - Lin J
AUID- ORCID: 0000-0002-1643-7421
AD  - The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, 
      Guangdong, China.
FAU - Li, Shasha
AU  - Li S
AUID- ORCID: 0000-0003-1170-4591
AD  - The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, 
      Guangdong, China.
FAU - Peng, Guiyuan
AU  - Peng G
AUID- ORCID: 0000-0003-3873-0845
AD  - Department of Otolaryngology, Guangdong Province Hospital of Chinese Medicine, 
      Guangzhou, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20210625
PL  - Egypt
TA  - J Oncol
JT  - Journal of oncology
JID - 101496537
PMC - PMC8257394
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2021/07/15 06:00
MHDA- 2021/07/15 06:01
PMCR- 2021/06/25
CRDT- 2021/07/14 07:02
PHST- 2020/09/10 00:00 [received]
PHST- 2021/06/08 00:00 [accepted]
PHST- 2021/07/14 07:02 [entrez]
PHST- 2021/07/15 06:00 [pubmed]
PHST- 2021/07/15 06:01 [medline]
PHST- 2021/06/25 00:00 [pmc-release]
AID - 10.1155/2021/8862821 [doi]
PST - epublish
SO  - J Oncol. 2021 Jun 25;2021:8862821. doi: 10.1155/2021/8862821. eCollection 2021.