PMID- 34257797
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 1939-4551 (Print)
IS  - 1939-4551 (Electronic)
IS  - 1939-4551 (Linking)
VI  - 14
IP  - 7
DP  - 2021 Jul
TI  - Allergenicity assessment and allergen profile analysis of different Chinese wheat 
      cultivars.
PG  - 100559
LID - 10.1016/j.waojou.2021.100559 [doi]
LID - 100559
AB  - BACKGROUNDS: As one of the most important cereals, wheat (Triticum aestivum) can 
      cause severe allergic reactions, such as baker's asthma, allergic rhinitis, and 
      atopic dermatitis. A growing number of people are developing allergies to Chinese 
      wheat; however, only a few wheat cultivars have been screened on allergenicity in 
      China. OBJECTIVE: The aim of the present study was to assess the allergenicity of 
      different Chinese wheat cultivars and characterize wheat allergen profiles of 
      patients with allergic rhinitis. METHODS: We determined protein (soluble protein, 
      gliadin, and glutenin) composition in Chinese wheat by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the immunoglobulin E 
      (IgE) binding capacity by enzyme-linked immunosorbent assay (ELISA) and Western 
      blot using 10 positive sera from wheat allergy patients. We identified 5 gel 
      bands with significant IgE binding capacity using liquid chromatography-tandem 
      mass spectrometry (LC-MS/MS). RESULTS: Soluble protein, albumin, and globulin, 
      showed the highest allergenicity, followed by gliadin, while glutenin only had 
      slight allergenicity. In soluble protein, 5 protein bands with molecular weights 
      of 27, 28, 53, 58, and 62 kDa showed very significant allergenicity. Meanwhile, 
      the relative abundances of 28 kDa-protein and 58 kDa-protein were significantly 
      positively correlated with the IgE-binding capacity of Chinese wheat cultivars, 
      which were identified as rRNA N-glycosidase and beta-amylase, respectively, among 
      other proteins in those highly complex gel bands. CONCLUSION AND CLINICAL 
      RELEVANCE: 28 kDa-protein (rRNA N-glycosidase) and 58 kDa-protein (beta-amylase) 
      were speculated to be the main allergens of Chinese wheat causing baker's asthma 
      and allergic rhinitis. These results provide new insights into the prevention and 
      treatment of wheat allergy and the development of hypoallergenic wheat products, 
      whose clinical significance is worth further evaluation.
CI  - (c) 2021 The Authors.
FAU - Wang, Yanbo
AU  - Wang Y
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Weng, Junjie
AU  - Weng J
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Zhu, Chengbo
AU  - Zhu C
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Ai, Rong
AU  - Ai R
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Zhou, Jinru
AU  - Zhou J
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Wang, Chong
AU  - Wang C
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Chen, Qing
AU  - Chen Q
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
FAU - Fu, Linglin
AU  - Fu L
AD  - Food Safety Key Laboratory of Zhejiang Province, School of Food Science and 
      Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
LA  - eng
PT  - Journal Article
DEP - 20210629
PL  - United States
TA  - World Allergy Organ J
JT  - The World Allergy Organization journal
JID - 101481283
PMC - PMC8258687
OTO - NOTNLM
OT  - Allergic rhinitis
OT  - Chinese wheat
OT  - Main allergens
OT  - Soluble protein
COIS- The authors declare that they have no conflict of interest.
EDAT- 2021/07/15 06:00
MHDA- 2021/07/15 06:01
PMCR- 2021/06/29
CRDT- 2021/07/14 07:04
PHST- 2020/10/09 00:00 [received]
PHST- 2021/05/07 00:00 [revised]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/07/14 07:04 [entrez]
PHST- 2021/07/15 06:00 [pubmed]
PHST- 2021/07/15 06:01 [medline]
PHST- 2021/06/29 00:00 [pmc-release]
AID - S1939-4551(21)00053-3 [pii]
AID - 100559 [pii]
AID - 10.1016/j.waojou.2021.100559 [doi]
PST - epublish
SO  - World Allergy Organ J. 2021 Jun 29;14(7):100559. doi: 
      10.1016/j.waojou.2021.100559. eCollection 2021 Jul.