PMID- 34262334
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 1178-7074 (Print)
IS  - 1178-7074 (Electronic)
IS  - 1178-7074 (Linking)
VI  - 14
DP  - 2021
TI  - Identification of an miRNA Regulatory Network and Candidate Markers for Ischemic 
      Stroke Related to Diabetes.
PG  - 3213-3223
LID - 10.2147/IJGM.S319503 [doi]
AB  - PURPOSE: Type 2 diabetes mellitus (T2DM) increases the risk of ischemic stroke 
      and poor prognosis. This study aimed to identify molecular mechanisms that are 
      dysregulated in T2DM-associated ischemic stroke and candidate genes that might 
      serve as biomarkers. METHODS: The top 25% variance genes in the GSE21321 and 
      GSE22255 datasets were analyzed for coexpression. The differentially expressed 
      mRNAs (DEmRs) between patients with T2DM or ischemic stroke and controls were 
      analyzed. Then, the union of overlapping coexpressed genes and overlapping DEmRs 
      was analyzed. The miRNAs differentially expressed in T2DM-associated ischemic 
      stroke were also analyzed. CIBERSORT was used to evaluate the levels of 
      infiltration by immune cells in T2DM-associated stroke. RESULTS: Thirteen 
      coexpression modules were identified in T2DM and 10 in ischemic stroke, and 594 
      module genes were shared between the two conditions. A total of 4452 mRNAs 
      differentially expressed between T2DM patients and controls were identified, as 
      were 2390 mRNAs differentially expressed between ischemic stroke and controls. 
      The 771 union genes were enriched mainly in immune-related biological functions 
      and signaling pathways. UBE2N, TGFB3, EXOSC1, and VIM were identified as 
      candidate markers. In addition, we identified miR-576-3p as having the most 
      regulatory roles in both T2DM and ischemic stroke. Mast cell activation was 
      significantly down-regulated in T2DM but up-regulated in ischemic stroke. 
      CONCLUSION: These findings provide numerous testable hypotheses about the 
      pathways underlying T2DM-associated ischemic stroke, which may help identify 
      therapeutic targets.
CI  - (c) 2021 Zhou et al.
FAU - Zhou, Hui
AU  - Zhou H
AD  - Department of Neurology, The People's Hospital of Guiping, Guiping, Guangxi, 
      537200, People's Republic of China.
FAU - Huang, Liujia
AU  - Huang L
AD  - Department of Rehabilitation Medicine, The People's Hospital of Guiping, Guiping, 
      Guangxi, 537200, People's Republic of China.
FAU - Liang, Lucong
AU  - Liang L
AD  - Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi, 530022, People's Republic of China.
FAU - Chen, Liechun
AU  - Chen L
AD  - Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi, 530022, People's Republic of China.
FAU - Zou, Chun
AU  - Zou C
AD  - Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi, 530022, People's Republic of China.
FAU - Li, Zhenhua
AU  - Li Z
AD  - Department of Emergency Medicine, The First People's Hospital of Nanning, 
      Nanning, 530022, People's Republic of China.
FAU - Li, Rongjie
AU  - Li R
AD  - Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi, 530022, People's Republic of China.
FAU - Jian, Chongdong
AU  - Jian C
AD  - Department of Neurology, The Affiliated Hospital of Youjiang Medical University 
      for Nationalities, Baise, Guangxi, 533000, People's Republic of China.
FAU - Zou, Donghua
AU  - Zou D
AUID- ORCID: 0000-0002-3891-9472
AD  - Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical 
      University, Nanning, Guangxi, 530022, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210707
PL  - New Zealand
TA  - Int J Gen Med
JT  - International journal of general medicine
JID - 101515487
PMC - PMC8274709
OTO - NOTNLM
OT  - candidate gene
OT  - immune response
OT  - ischemic stroke
OT  - miRNAs
OT  - type 2 diabetes mellitus
COIS- The authors declare that this research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/16 06:00
MHDA- 2021/07/16 06:01
PMCR- 2021/07/07
CRDT- 2021/07/15 06:17
PHST- 2021/05/12 00:00 [received]
PHST- 2021/06/29 00:00 [accepted]
PHST- 2021/07/15 06:17 [entrez]
PHST- 2021/07/16 06:00 [pubmed]
PHST- 2021/07/16 06:01 [medline]
PHST- 2021/07/07 00:00 [pmc-release]
AID - 319503 [pii]
AID - 10.2147/IJGM.S319503 [doi]
PST - epublish
SO  - Int J Gen Med. 2021 Jul 7;14:3213-3223. doi: 10.2147/IJGM.S319503. eCollection 
      2021.