PMID- 34262705 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240402 IS - 2048-7177 (Print) IS - 2048-7177 (Electronic) IS - 2048-7177 (Linking) VI - 9 IP - 7 DP - 2021 Jul TI - Lactoferrin could alleviate liver injury caused by Maillard reaction products with furan ring through regulating necroptosis pathway. PG - 3449-3459 LID - 10.1002/fsn3.2254 [doi] AB - As classical MRPs, the toxic effects of furosine, pyralline, and 5-hydroxymethylfurfural (5-HMF) in liver tissue are evaluated and the related mechanism is investigated here, and the protective effects of lactoferrin on liver injury caused by Maillard reaction products (MRPs) with furan ring are proved in vitro and in vivo. First, we detect the concentrations of furosine, pyralline, and 5-HMF in several foods using ultrahigh-performance liquid chromatography (UHPLC). Then, the effects of the three MRPs on liver cells (HL-7702) viability, as well as liver tissue, are performed and evaluated. Furthermore, the regulations of three MRPs on necroptosis-related pathway in liver cells are investigated. Additionally, the effects of lactoferrin in alleviating liver injury, as well as regulating necroptosis pathway, were evaluated. Results elucidate that lactoferrin protects liver injury caused by MRPs with furan ring structure through activating RIPK1/RIPK3/p-MLKL necroptosis pathway and downstream inflammatory reaction. CI - (c) 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. FAU - Fan, Linlin AU - Fan L AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Wang, Fengen AU - Wang F AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Yao, Qianqian AU - Yao Q AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Wu, Haoming AU - Wu H AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Wen, Fang AU - Wen F AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Wang, Jiaqi AU - Wang J AUID- ORCID: 0000-0001-8841-0124 AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Li, Huiying AU - Li H AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. FAU - Zheng, Nan AU - Zheng N AUID- ORCID: 0000-0002-8469-4243 AD - Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. AD - Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing China. AD - State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China. LA - eng PT - Journal Article DEP - 20210506 PL - United States TA - Food Sci Nutr JT - Food science & nutrition JID - 101605473 PMC - PMC8269604 OTO - NOTNLM OT - 5-hydroxymethylfurfural (5-HMF) OT - Maillard reaction products (MRPs) OT - furosine OT - lactoferrin OT - pyralline COIS- The authors declare that they do not have any conflict of interest. EDAT- 2021/07/16 06:00 MHDA- 2021/07/16 06:01 PMCR- 2021/05/06 CRDT- 2021/07/15 06:21 PHST- 2020/10/30 00:00 [received] PHST- 2021/03/09 00:00 [revised] PHST- 2021/03/11 00:00 [accepted] PHST- 2021/07/15 06:21 [entrez] PHST- 2021/07/16 06:00 [pubmed] PHST- 2021/07/16 06:01 [medline] PHST- 2021/05/06 00:00 [pmc-release] AID - FSN32254 [pii] AID - 10.1002/fsn3.2254 [doi] PST - epublish SO - Food Sci Nutr. 2021 May 6;9(7):3449-3459. doi: 10.1002/fsn3.2254. eCollection 2021 Jul.