PMID- 34262867
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210716
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - The Proton-Boron Reaction Increases the Radiobiological Effectiveness of Clinical 
      Low- and High-Energy Proton Beams: Novel Experimental Evidence and Perspectives.
PG  - 682647
LID - 10.3389/fonc.2021.682647 [doi]
LID - 682647
AB  - Protontherapy is a rapidly expanding radiotherapy modality where accelerated 
      proton beams are used to precisely deliver the dose to the tumor target but is 
      generally considered ineffective against radioresistant tumors. Proton-Boron 
      Capture Therapy (PBCT) is a novel approach aimed at enhancing proton biological 
      effectiveness. PBCT exploits a nuclear fusion reaction between low-energy protons 
      and (11)B atoms, i.e. p+(11)B--> 3alpha (p-B), which is supposed to produce highly-DNA 
      damaging alpha-particles exclusively across the tumor-conformed Spread-Out Bragg Peak 
      (SOBP), without harming healthy tissues in the beam entrance channel. To confirm 
      previous work on PBCT, here we report new in-vitro data obtained at the 62-MeV 
      ocular melanoma-dedicated proton beamline of the INFN-Laboratori Nazionali del 
      Sud (LNS), Catania, Italy. For the first time, we also tested PBCT at the 250-MeV 
      proton beamline used for deep-seated cancers at the Centro Nazionale di 
      Adroterapia Oncologica (CNAO), Pavia, Italy. We used Sodium Mercaptododecaborate 
      (BSH) as (11)B carrier, DU145 prostate cancer cells to assess cell killing and 
      non-cancer epithelial breast MCF-10A cells for quantifying chromosome aberrations 
      (CAs) by FISH painting and DNA repair pathway protein expression by western 
      blotting. Cells were exposed at various depths along the two clinical SOBPs. 
      Compared to exposure in the absence of boron, proton irradiation in the presence 
      of BSH significantly reduced DU145 clonogenic survival and increased both 
      frequency and complexity of CAs in MCF-10A cells at the mid- and distal SOBP 
      positions, but not at the beam entrance. BSH-mediated enhancement of DNA damage 
      response was also found at mid-SOBP. These results corroborate PBCT as a strategy 
      to render protontherapy amenable towards radiotherapy-resilient tumor. If coupled 
      with emerging proton FLASH radiotherapy modalities, PBCT could thus widen the 
      protontherapy therapeutic index.
CI  - Copyright (c) 2021 Blaha, Feoli, Agosteo, Calvaruso, Cammarata, Catalano, Ciocca, 
      Cirrone, Conte, Cuttone, Facoetti, Forte, Giuffrida, Magro, Margarone, Minafra, 
      Petringa, Pucci, Ricciardi, Rosa, Russo and Manti.
FAU - Blaha, Pavel
AU  - Blaha P
AD  - Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Napoli, Naples, Italy.
FAU - Feoli, Chiara
AU  - Feoli C
AD  - Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Napoli, Naples, Italy.
FAU - Agosteo, Stefano
AU  - Agosteo S
AD  - Energy Department, Politecnico di Milano, and INFN, Sezione di Milano, Milan, 
      Italy.
FAU - Calvaruso, Marco
AU  - Calvaruso M
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Cammarata, Francesco Paolo
AU  - Cammarata FP
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Catalano, Roberto
AU  - Catalano R
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Ciocca, Mario
AU  - Ciocca M
AD  - Medical Physics Unit & Research Department, Centro Nazionale di Adroterapia 
      Oncologica (CNAO) & INFN, Sezione di Pavia, Pavia, Italy.
FAU - Cirrone, Giuseppe Antonio Pablo
AU  - Cirrone GAP
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Conte, Valeria
AU  - Conte V
AD  - Laboratori Nazionali di Legnaro (LNL), INFN, Legnaro, Italy.
FAU - Cuttone, Giacomo
AU  - Cuttone G
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Facoetti, Angelica
AU  - Facoetti A
AD  - Medical Physics Unit & Research Department, Centro Nazionale di Adroterapia 
      Oncologica (CNAO) & INFN, Sezione di Pavia, Pavia, Italy.
FAU - Forte, Giusi Irma
AU  - Forte GI
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Giuffrida, Lorenzo
AU  - Giuffrida L
AD  - Extreme Light Infrastructure (ELI)-Beamlines Center, Institute of Physics (FZU), 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Magro, Giuseppe
AU  - Magro G
AD  - Medical Physics Unit & Research Department, Centro Nazionale di Adroterapia 
      Oncologica (CNAO) & INFN, Sezione di Pavia, Pavia, Italy.
FAU - Margarone, Daniele
AU  - Margarone D
AD  - Extreme Light Infrastructure (ELI)-Beamlines Center, Institute of Physics (FZU), 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Minafra, Luigi
AU  - Minafra L
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
FAU - Petringa, Giada
AU  - Petringa G
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
AD  - Extreme Light Infrastructure (ELI)-Beamlines Center, Institute of Physics (FZU), 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Pucci, Gaia
AU  - Pucci G
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies 
      (STeBiCeF), Universita di Palermo, Palermo, Italy.
FAU - Ricciardi, Valerio
AU  - Ricciardi V
AD  - Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Napoli, Naples, Italy.
AD  - Department of Mathematics & Physics, Universita L. Vanvitelli, Caserta, Italy.
FAU - Rosa, Enrico
AU  - Rosa E
AD  - Radiation Biophysics Laboratory, Department of Physics "E. Pancini", Universita 
      di Napoli Federico II, Naples, Italy.
FAU - Russo, Giorgio
AU  - Russo G
AD  - Istituto di Bioimmagini e Fisiologia Molecolare-Consiglio Nazionale delle 
      Ricerche (IBFM-CNR), Cefalu, Italy.
AD  - Laboratori Nazionali del Sud (LNS), INFN, Catania, Italy.
AD  - The Sicilian Center of Nuclear Physics and the Structure of Matter (CSFNSM), 
      Catania, Italy.
FAU - Manti, Lorenzo
AU  - Manti L
AD  - Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Napoli, Naples, Italy.
AD  - Radiation Biophysics Laboratory, Department of Physics "E. Pancini", Universita 
      di Napoli Federico II, Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210628
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8274279
OTO - NOTNLM
OT  - BSH
OT  - alpha-particle
OT  - cancer cell killing
OT  - chromosome aberrations
OT  - proton-boron (B) fusion-enhanced proton therapy (PBFEPT)
OT  - protontherapy
COIS- Author LG, DM and GAPC declare a potential conflict of interest being patent 
      holder of the following invention: "DEVICE AND METHOD FOR IMAGING AND ENHANCED 
      PROTONTHERAPY TREATMENT USING NUCLEAR REACTIONS. Application no.: EP16178280.0 - 
      1109/3266470. The remaining authors declare that the work described in this paper 
      was conducted in the absence of any specific relationship that could be construed 
      as a potential conflict of interest.
EDAT- 2021/07/16 06:00
MHDA- 2021/07/16 06:01
PMCR- 2021/01/01
CRDT- 2021/07/15 06:23
PHST- 2021/03/18 00:00 [received]
PHST- 2021/05/17 00:00 [accepted]
PHST- 2021/07/15 06:23 [entrez]
PHST- 2021/07/16 06:00 [pubmed]
PHST- 2021/07/16 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.682647 [doi]
PST - epublish
SO  - Front Oncol. 2021 Jun 28;11:682647. doi: 10.3389/fonc.2021.682647. eCollection 
      2021.