PMID- 34265416
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20210826
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 116
DP  - 2021 Sep
TI  - Grass carp (Ctenopharyngodon idellus) SHP2 suppresses IFN I expression via 
      decreasing the phosphorylation of GSK3beta in a non-contact manner.
PG  - 150-160
LID - S1050-4648(21)00194-7 [pii]
LID - 10.1016/j.fsi.2021.07.005 [doi]
AB  - As a tyrosine phosphatase, Src homology 2-containing protein tyrosine phosphatase 
      2 (SHP2) serves as an inhibitor in PI3K-Akt pathway. In mammals, SHP2 can 
      phosphorylate GSK3beta at Y216 site to control the expression of IFN. So far, the 
      multiple functions of SHP2 have been reported in mammals. However, little is 
      known about fish SHP2. In this study, we cloned and identified a grass carp 
      (Ctenopharyngodon idellus) SHP2 gene (CiSHP2, MT373151). SHP2 is conserved among 
      different vertebrates by amino acid sequences alignment and the phylogenetic tree 
      analysis. CiSHP2 shared the closest homology with Danio rerio SHP2. 
      Simultaneously, SHP2 was also tested in grass carp tissues and CIK (C. idellus 
      kidney) cells. We found that it responded to poly I:C stimulation. CiSHP2 was 
      located in the cytoplasm just as the same as those of mammals. Interestingly, it 
      inhibited the phosphorylation level of GSK3beta in a non-contact manner. Meanwhile 
      CiGSK3beta interacted with and directly phosphorylated CiTBK1. In addition, we found 
      that CiSHP2 also reduced the phosphorylation level of CiTBK1 by CiGSK3beta, and then 
      it depressed the expression of IFN I via GSK3beta-TBK1 axis. These results suggested 
      that CiSHP2 was involved in CiGSK3beta and CiTBK1 activity but not regulated their 
      transcriptional level. At the same time, we also found that CiSHP2 also 
      influenced the activity of CiIRF3. Therefore, fish SHP2 inhibited IFN I 
      expression through blocking GSK3beta-TBK1 signal axis.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Lu, Shina
AU  - Lu S
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Peng, Xiaojue
AU  - Peng X
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Lin, Gang
AU  - Lin G
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Xu, Kang
AU  - Xu K
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Wang, Shanghong
AU  - Wang S
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Qiu, Weihua
AU  - Qiu W
AD  - Teaching Material Research Office of Jiangxi Provincial Education Department, 
      Nanchang, 330046, Jiangxi, China.
FAU - Du, Hailing
AU  - Du H
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Chang, Kaile
AU  - Chang K
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Lv, Yangfeng
AU  - Lv Y
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Liu, Yapeng
AU  - Liu Y
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Deng, Hang
AU  - Deng H
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Hu, Chengyu
AU  - Hu C
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China.
FAU - Xu, Xiaowen
AU  - Xu X
AD  - School of Life Science, Nanchang University, Nanchang, 330031, Jiangxi, China. 
      Electronic address: xuxw2020@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210712
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Interferon Type I)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carps/genetics/*immunology
MH  - Cell Line
MH  - Fish Proteins/genetics/*immunology
MH  - Glycogen Synthase Kinase 3 beta/*immunology
MH  - Interferon Type I/*immunology
MH  - Phosphorylation
MH  - Phylogeny
MH  - Poly I-C/pharmacology
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics/*immunology
OTO - NOTNLM
OT  - Fish
OT  - GSK3beta-TBK1 axis
OT  - Innate immune
OT  - Phosphorylation
OT  - SHP2
EDAT- 2021/07/16 06:00
MHDA- 2021/08/27 06:00
CRDT- 2021/07/15 20:16
PHST- 2020/12/16 00:00 [received]
PHST- 2021/07/05 00:00 [revised]
PHST- 2021/07/11 00:00 [accepted]
PHST- 2021/07/16 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2021/07/15 20:16 [entrez]
AID - S1050-4648(21)00194-7 [pii]
AID - 10.1016/j.fsi.2021.07.005 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2021 Sep;116:150-160. doi: 10.1016/j.fsi.2021.07.005. 
      Epub 2021 Jul 12.