PMID- 34265754
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 16
IP  - 5
DP  - 2021 Jul 29
TI  - 3D-printed biphasic scaffolds for the simultaneous regeneration of osteochondral 
      tissues.
LID - 10.1088/1748-605X/ac14cb [doi]
AB  - Osteochondral tissue engineering (OCTE) involves the simulation of highly complex 
      tissues with disparate biomechanical properties. OCTE is regarded as the best 
      option for treating osteochondral defects, most of the drawbacks of current 
      treatment methodologies can be addressed by this method. In recent years, the 
      conventional scaffolds used in cartilage and bone regeneration are gradually 
      being replaced by 3D printed scaffolds (3DP). In the present study, we devised 
      the strategy of 3D printing for fabricating biphasic and integrated scaffolds 
      that are loaded with bioactive factors for enhancing the osteochondral tissue 
      regeneration. Polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA), is 
      used along with bioactive factors (chondroitin sulphate and beta-tricalcium 
      phosphate (betaTCP)) for the upper cartilage and lower bone layer respectively. The 
      3D printed bi-layered scaffolds with varying infill density, to mimic the native 
      tissue, are not previously explored for OCTE. Hence, we tested the simultaneous 
      osteochondrogenic differentiation inducing potential of the aforesaid 3D printed 
      biphasic scaffoldsin vitro, using rabbit adipose derived mesenchymal stem cells 
      (ADMSCs). Further, the biphasic scaffolds were highly cytocompatible, with 
      excellent cell adhesion properties and cellular morphology. Most importantly, 
      these biphasic scaffolds directed the simultaneous differentiation of a single 
      stem cell population in to two cell lineages (simultaneous differentiation of 
      rabbit ADMSCs into chondrocytes and osteoblasts). Further, these scaffolds 
      enhanced the production of ECM and induced robust expression of marker genes that 
      is specific for respective cartilage and bone layers. The 3D printed OCTE 
      scaffold of our study hence can simulate the native osteochondral unit and could 
      be potential futuristic biomimetic scaffold for osteochondral defects. Furtherin 
      vivostudies are warranted.
CI  - (c) 2021 IOP Publishing Ltd.
FAU - Natarajan, Amrita Bds MTech
AU  - Natarajan ABM
AUID- ORCID: 0000-0001-9149-6045
AD  - Division of Tissue Engineering and Regeneration Technologies, Department of 
      Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute of 
      Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
FAU - Sivadas, Vp Ph D
AU  - Sivadas VPD
AUID- ORCID: 0000-0002-3562-8493
AD  - Division of Tissue Engineering and Regeneration Technologies, Department of 
      Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute of 
      Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
FAU - Nair, Prabha D Ph D
AU  - Nair PDPD
AUID- ORCID: 0000-0003-4263-1575
AD  - Division of Tissue Engineering and Regeneration Technologies, Department of 
      Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute of 
      Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210729
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
SB  - IM
MH  - Animals
MH  - *Bone Regeneration
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - *Chondrogenesis
MH  - Mesenchymal Stem Cells/cytology/physiology
MH  - *Osteogenesis
MH  - *Printing, Three-Dimensional
MH  - Rabbits
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - 3D printed scaffolds
OT  - 3D printing
OT  - biphasic
OT  - chondrogenesis
OT  - osteochondral
OT  - osteogenesis
EDAT- 2021/07/16 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/07/15 20:32
PHST- 2021/03/14 00:00 [received]
PHST- 2021/07/15 00:00 [accepted]
PHST- 2021/07/16 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/07/15 20:32 [entrez]
AID - 10.1088/1748-605X/ac14cb [doi]
PST - epublish
SO  - Biomed Mater. 2021 Jul 29;16(5). doi: 10.1088/1748-605X/ac14cb.